The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of MPTP-lesioned macaques and in the cerebrospinal fluid of Parkinson's disease patients.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
20 06 2019
Historique:
received: 07 03 2019
accepted: 03 06 2019
entrez: 22 6 2019
pubmed: 22 6 2019
medline: 28 10 2020
Statut: epublish

Résumé

Dysfunction of NMDA receptor (NMDAR)-mediated transmission is supposed to contribute to the motor and non-motor symptoms of Parkinson's Disease (PD), and to L-DOPA-induced dyskinesia. Besides the main agonist L-glutamate, two other amino acids in the atypical D-configuration, D-serine and D-aspartate, activate NMDARs. In the present work, we investigated the effect of dopamine depletion on D-amino acids metabolism in the brain of MPTP-lesioned Macaca mulatta, and in the serum and cerebrospinal fluid of PD patients. We found that MPTP treatment increases D-aspartate and D-serine in the monkey putamen while L-DOPA rescues both D-amino acids levels. Conversely, dopaminergic denervation is associated with selective D-serine reduction in the substantia nigra. Such decrease suggests that the beneficial effect of D-serine adjuvant therapy previously reported in PD patients may derive from the normalization of endogenous D-serine levels and consequent improvement of nigrostriatal hypoglutamatergic transmission at glycine binding site. We also found reduced D-serine concentration in the cerebrospinal fluid of L-DOPA-free PD patients. These results further confirm the existence of deep interaction between dopaminergic and glutamatergic neurotransmission in PD and disclose a possible direct influence of D-amino acids variations in the changes of NMDAR transmission occurring under dopamine denervation and L-DOPA therapy.

Identifiants

pubmed: 31222058
doi: 10.1038/s41598-019-45419-1
pii: 10.1038/s41598-019-45419-1
pmc: PMC6586824
doi:

Substances chimiques

Receptors, N-Methyl-D-Aspartate 0
Serine 452VLY9402

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8898

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Auteurs

Tommaso Nuzzo (T)

Translational Neuroscience Unit, IRCCS Casa Sollievo della Sofferenza, 71013, San Giovanni Rotondo, Italy.

Daniela Punzo (D)

Laboratory of Behavioural Neuroscience, Ceinge Biotecnologie Avanzate, 80145, Naples, Italy.
Department of Environmental, Biological and Pharmaceutical Science and Technologies, Università degli Studi della Campania "Luigi Vanvitelli", 81100, Caserta, Italy.

Paola Devoto (P)

Department of Biomedical Sciences, University of Cagliari, 09042, Monserrato, Italy.

Elena Rosini (E)

Department of Biotechnology and Life Sciences, Università degli Studi dell'Insubria, 21100, Varese, Italy.

Silvia Paciotti (S)

Department of Pharmaceutical Sciences, University of Perugia, 06122, Perugia, Italy.

Silvia Sacchi (S)

Department of Biotechnology and Life Sciences, Università degli Studi dell'Insubria, 21100, Varese, Italy.

Qin Li (Q)

Motac Neuroscience, UK-M15 6WE, Manchester, United Kingdom.
Institute of Lab Animal Sciences, China Academy of Medical Sciences, Beijing, China.

Marie-Laure Thiolat (ML)

Université de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France.
Centre National de la Recherche Scientifique Unité Mixte de Recherche 5293, Institut des Maladies Neurodégénératives, Bordeaux, France.

Celine Véga (C)

Université de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France.

Massimo Carella (M)

Translational Neuroscience Unit, IRCCS Casa Sollievo della Sofferenza, 71013, San Giovanni Rotondo, Italy.

Manolo Carta (M)

Department of Biomedical Sciences, University of Cagliari, 09042, Monserrato, Italy.

Fabrizio Gardoni (F)

Dipartimento di Scienze Farmacologiche e Biomolecolari (DiSFeB), Università degli Studi di Milano "La Statale", 20133, Milan, Italy.

Paolo Calabresi (P)

Department of Medicine, Neurology Clinic, University Hospital of Perugia, 06129, Perugia, Italy.

Loredano Pollegioni (L)

Department of Biotechnology and Life Sciences, Università degli Studi dell'Insubria, 21100, Varese, Italy.

Erwan Bezard (E)

Motac Neuroscience, UK-M15 6WE, Manchester, United Kingdom.
Institute of Lab Animal Sciences, China Academy of Medical Sciences, Beijing, China.
Université de Bordeaux, Institut des Maladies Neurodégénératives, Bordeaux, France.
Centre National de la Recherche Scientifique Unité Mixte de Recherche 5293, Institut des Maladies Neurodégénératives, Bordeaux, France.

Lucilla Parnetti (L)

Department of Medicine, Neurology Clinic, University Hospital of Perugia, 06129, Perugia, Italy.

Francesco Errico (F)

Department of Agricultural Sciences, University of Naples "Federico II", 80055, Portici, Italy. francesco.errico@unina.it.

Alessandro Usiello (A)

Laboratory of Behavioural Neuroscience, Ceinge Biotecnologie Avanzate, 80145, Naples, Italy. usiello@ceinge.unina.it.
Department of Environmental, Biological and Pharmaceutical Science and Technologies, Università degli Studi della Campania "Luigi Vanvitelli", 81100, Caserta, Italy. usiello@ceinge.unina.it.

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