Is sex a biological variable in corneal wound healing?
Actins
/ genetics
Animals
Burns, Chemical
/ genetics
Collagen Type I
/ genetics
Corneal Injuries
/ genetics
Eye Burns
/ chemically induced
Fibronectins
/ genetics
Fluorescent Antibody Technique
In Situ Nick-End Labeling
RNA, Messenger
/ genetics
Rabbits
Real-Time Polymerase Chain Reaction
Sex Factors
Sodium Hydroxide
/ toxicity
Transforming Growth Factor beta1
/ genetics
Wound Healing
/ physiology
Cornea
Fibrosis
Male vs. female
Sex variable
Wound healing
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
25
03
2019
revised:
16
06
2019
accepted:
17
06
2019
pubmed:
22
6
2019
medline:
29
2
2020
entrez:
22
6
2019
Statut:
ppublish
Résumé
Wound healing differs significantly between men and women in a tissue-dependent manner. Dermal wounds heal faster in women whereas mucosal wounds heal faster in men. However, the effect of sex as a variable in corneal wound healing is largely unknown. The primary objective of this study was to test whether sex is a biological variable in corneal wound healing activated by the trauma or injury using an established in vivo rabbit model with male and female New Zealand White rabbits. Corneal wounds in rabbits were produced by a single topical alkali (0.5N Sodium hydroxide) application. Serial slit-lamp, stereo biomicroscopy, and applanation tonometry evaluated corneal opacity, anterior segment ocular health, and intraocular pressure (IOP), respectively, at various times during the study. Fourteen days after alkali-wound, corneal tissues were collected after humane euthanasia to examine cellular and molecular wound healing parameters. Quantitative PCR (qPCR) and immunofluorescence were used to quantify changes in the extracellular modeling protein levels of alpha-smooth muscle actin (α-SMA), Fibronectin (FN), Collagen-I (Col-I), and Transforming growth factor beta 1 (TGFβ1) involved in corneal healing. Hematoxylin and Eosin (H&E) staining was used to study histopathological changes in morphology and TUNEL assay to evaluate levels of apoptotic cell death. Male and female rabbits showed no significant differences in corneal opacity (Fantes score) or intraocular pressure (IOP) values (9.5 ± 0.5 mm Hg) in live animals. Likewise, no statistically significant sex-based differences in the mRNA levels of α-SMA (male = 5.95 ± 0.21 fold vs. female = 5.32 ± 0.043), FN (male = 3.02 ± 0.24 fold vs. female = 3.23 ± 0.27), Col-I (male = 3.12 ± 0.37 fold vs. female = 3.31 ± 0.24), TGFβ1 (male = 1.65 ± 0.06 fold vs. female = 1.59 ± 0.053); and protein levels of α-SMA (male = 74.16 ± 4.6 vs. female = 71.58 ± 7.1), FN (male = 60.11 ± 4.6 vs. female = 57.41 ± 8.3), Col-I (male = 84.11 ± 2.8 vs. female = 84.55 ± 3.6), TGFβ1 (male = 11.61 ± 2.8 vs. female = 9.5 ± 3.04) were observed. Furthermore, H&E and TUNEL analyses found no statistically significant differences in cellular structures and apoptosis, respectively, in male vs. female corneas. Consistent with earlier reports, wounded corneas showed significantly increased levels of these parameters compared to the unwounded corneas. Our data suggest that sex is not a major biological variable during active early stages of corneal wound healing in rabbits in vivo.
Identifiants
pubmed: 31226339
pii: S0014-4835(19)30099-5
doi: 10.1016/j.exer.2019.107705
pmc: PMC9250696
mid: NIHMS1817617
pii:
doi:
Substances chimiques
Actins
0
Collagen Type I
0
Fibronectins
0
RNA, Messenger
0
Transforming Growth Factor beta1
0
Sodium Hydroxide
55X04QC32I
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
107705Subventions
Organisme : BLRD VA
ID : I01 BX000357
Pays : United States
Organisme : BLRD VA
ID : IK6 BX005646
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY017294
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030774
Pays : United States
Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.
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