Effects of adipose and bone marrow-derived mesenchymal stem cells on vaginal atrophy in a rat menopause model.
Adipose Tissue
/ cytology
Animals
Atrophy
Biomarkers
/ metabolism
Bone Marrow Cells
/ cytology
Cells, Cultured
Disease Models, Animal
Estrogen Receptor alpha
/ metabolism
Female
Humans
Menopause
/ metabolism
Mesenchymal Stem Cell Transplantation
/ methods
Mesenchymal Stem Cells
/ cytology
Rats
Vagina
/ metabolism
Vascular Endothelial Growth Factor A
/ metabolism
Vascular Endothelial Growth Factor Receptor-1
/ metabolism
bcl-2-Associated X Protein
/ metabolism
Adipogenic derived mesenchymal stem cells
Bone marrow derived mesenchymal stem cells
Menopause
Rat
Vaginal atrophy
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
30 Aug 2019
30 Aug 2019
Historique:
received:
12
02
2019
revised:
03
06
2019
accepted:
13
06
2019
pubmed:
23
6
2019
medline:
30
7
2019
entrez:
23
6
2019
Statut:
ppublish
Résumé
Vaginal atrophy is characterized by thinning of vaginal epithelial layers and decreased local blood flow. We aimed to evaluate the regenerative effects of Adipose derived mesenchymal stem cells (ADMSC) and Bone marrow derived mesenchymal stem cells (BMDSC) on vaginal atrophy in rat menopause model. Rats were randomly divided into 4 (four) groups: sham, control, ADMSC, BMDSC. Vaginal epithelial thickness, structure of the lamina propria, blood vessels in the lamina propria, collagen deposition, and muscle structure were evaluated. Anti ER α, VEGF, VEGFR 1, Bax and bcl-2 antibodies were analyzed. Beta actin gene was used as endogenous control. Genetical differences among the groups were compared by using Kruskal Wallis and Mann Whitney U test. p < 0.05 was regarded as statistically significant. Epithelial thickness of ADMSC group was higher than control group, but less than sham group Epithelial thickness of BMDSC group was less than sham group. Lamina propria and muscle tissue of ADMSC and BMDSC groups were found to be similar to sham group. VEGFR-1, VEGF, Bax and ER-α staining levels were higher in ADMSC and BMDSC groups than control group. ADMSC group stained stronger with VEGFR-1 and VEGF than BMDSC group. Bcl-2 staining level was increased in ADMSC applied group. No statistically significant difference was detected in Bax and Bcl-2 genes and Bax-/Bcl-2 ratio. Although genetic expression might have ended and could not be significantly demonstrated, histological and immunohistochemical results favor ADMSC application in vaginal atrophy rather than BMDSC.
Sections du résumé
BACKGROUND & OBJECTIVES
OBJECTIVE
Vaginal atrophy is characterized by thinning of vaginal epithelial layers and decreased local blood flow. We aimed to evaluate the regenerative effects of Adipose derived mesenchymal stem cells (ADMSC) and Bone marrow derived mesenchymal stem cells (BMDSC) on vaginal atrophy in rat menopause model.
MATERIALS AND METHODS
METHODS
Rats were randomly divided into 4 (four) groups: sham, control, ADMSC, BMDSC. Vaginal epithelial thickness, structure of the lamina propria, blood vessels in the lamina propria, collagen deposition, and muscle structure were evaluated. Anti ER α, VEGF, VEGFR 1, Bax and bcl-2 antibodies were analyzed. Beta actin gene was used as endogenous control. Genetical differences among the groups were compared by using Kruskal Wallis and Mann Whitney U test. p < 0.05 was regarded as statistically significant.
RESULTS
RESULTS
Epithelial thickness of ADMSC group was higher than control group, but less than sham group Epithelial thickness of BMDSC group was less than sham group. Lamina propria and muscle tissue of ADMSC and BMDSC groups were found to be similar to sham group. VEGFR-1, VEGF, Bax and ER-α staining levels were higher in ADMSC and BMDSC groups than control group. ADMSC group stained stronger with VEGFR-1 and VEGF than BMDSC group. Bcl-2 staining level was increased in ADMSC applied group. No statistically significant difference was detected in Bax and Bcl-2 genes and Bax-/Bcl-2 ratio.
CONCLUSIONS
CONCLUSIONS
Although genetic expression might have ended and could not be significantly demonstrated, histological and immunohistochemical results favor ADMSC application in vaginal atrophy rather than BMDSC.
Identifiants
pubmed: 31228541
pii: S0378-1119(19)30587-6
doi: 10.1016/j.gene.2019.06.027
pii:
doi:
Substances chimiques
Bax protein, mouse
0
Biomarkers
0
Estrogen Receptor alpha
0
Vascular Endothelial Growth Factor A
0
bcl-2-Associated X Protein
0
vascular endothelial growth factor A, mouse
0
Vascular Endothelial Growth Factor Receptor-1
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Pagination
143937Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.