Genetic manipulation allows in vivo tracking of the life cycle of the son-killer symbiont, Arsenophonus nasoniae, and reveals patterns of host invasion, tropism and pathology.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
06
03
2019
revised:
19
06
2019
accepted:
21
06
2019
pubmed:
27
6
2019
medline:
10
5
2020
entrez:
26
6
2019
Statut:
ppublish
Résumé
Maternally heritable symbionts are common in arthropods and represent important partners and antagonists. A major impediment to understanding the mechanistic basis of these symbioses has been lack of genetic manipulation tools, for instance, those enabling transgenic GFP expression systems for in vivo visualization. Here, we transform the 'son-killer' reproductive parasite Arsenophonus nasoniae that infects the parasitic wasp Nasonia vitripennis with the plasmid pOM1-gfp, re-introduce this strain to N. vitripennis and then used this system to track symbiont life history in vivo. These data revealed transfer of the symbiont into the fly pupa by N. vitripennis during oviposition and N. vitripennis larvae developing infection over time through feeding. A strong tropism of A. nasoniae to the N. vitripennis ovipositor developed during wasp pupation, which aids onward transmission. The symbiont was also visualized in diapause larvae. Occasional necrotic diapause larvae were observed which displayed intense systemic infection alongside widespread melanotic nodules indicative of an active but failed immune response. Our results provide the foundation for the study of this symbiosis through in vivo tracking of the fate of symbionts through host development, which is rarely achieved in heritable microbe/insect interactions.
Identifiants
pubmed: 31237728
doi: 10.1111/1462-2920.14724
pmc: PMC6771839
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3172-3182Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 708232
Pays : International
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 704382
Pays : International
Organisme : Natural Environment Research Council
ID : NE/I01067X/1
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L014947/1
Pays : United Kingdom
Informations de copyright
© 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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