Formation of 3-hydroxyglutaric acid in glutaric aciduria type I: in vitro participation of medium chain acyl-CoA dehydrogenase.

3‐hydroxyglutaric acid acyl‐CoA dehydrogenase glutaconyl‐CoA glutaric aciduria type I glutaryl‐CoA

Journal

JIMD reports
ISSN: 2192-8304
Titre abrégé: JIMD Rep
Pays: United States
ID NLM: 101568557

Informations de publication

Date de publication:
May 2019
Historique:
received: 26 11 2018
accepted: 28 01 2019
entrez: 27 6 2019
pubmed: 27 6 2019
medline: 27 6 2019
Statut: epublish

Résumé

3-Hydroxyglutaric acid (3-OH-GA) in urine has been identified as the most reliable diagnostic marker for glutaric aciduria type I (GA I). We showed that hydratation of glutaconyl-CoA to 3-hydroxyglutaryl-CoA, which is subsequently hydrolyzed to 3-OH-GA, is efficiently catalyzed by 3-methylglutaconyl-CoA hydratase (3-MGH). We have now investigated whether mitochondrial acyl-CoA-dehydrogenases can convert glutaryl-CoA to glutaconyl-CoA. Short-chain acyl-CoA dehydrogenase (SCAD), medium-chain acyl-CoA dehydrogenase (MCAD), and long-chain acyl-CoA dehydrogenase (LCAD) accepted glutaryl-CoA as a substrate. The highest

Identifiants

pubmed: 31240164
doi: 10.1002/jmd2.12026
pii: JMD212026
pmc: PMC6498835
doi:

Types de publication

Journal Article

Langues

eng

Pagination

30-34

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK109907
Pays : United States

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Auteurs

Verena Peters (V)

Center für Paediatric and Adolescent Medicine University of Heidelberg Germany.

Marina Morath (M)

Center für Paediatric and Adolescent Medicine University of Heidelberg Germany.

Matthias Mack (M)

Institut für Technische Mikrobiologie Hochschule Mannheim Germany.

Michael Liesert (M)

Laboratorium für Mikrobiologie, Fachbereich Biologie Philipps-Universität Marburg Germany.

Wolfgang Buckel (W)

Laboratorium für Mikrobiologie, Fachbereich Biologie Philipps-Universität Marburg Germany.

Georg F Hoffmann (GF)

Center für Paediatric and Adolescent Medicine University of Heidelberg Germany.

Jerry Vockley (J)

Department of Pediatric and Human Genetics Children's Hospital of Pittsburgh and University of Pittsburgh Pittsburgh Pennsylvania.

Sandro Ghisla (S)

Department of Biology, Section of Natural Sciences Universität Konstanz Germany.

Johannes Zschocke (J)

Division of Human Genetics Medical University Innsbruck Austria.

Classifications MeSH