Two large reciprocal translocations characterized in the disease resistance-rich burmannica genetic group of Musa acuminata.
Musa acuminata
banana
chromosomal rearrangement
genotyping by sequencing
paired-end sequencing
reciprocal translocation
Journal
Annals of botany
ISSN: 1095-8290
Titre abrégé: Ann Bot
Pays: England
ID NLM: 0372347
Informations de publication
Date de publication:
24 09 2019
24 09 2019
Historique:
received:
18
02
2019
accepted:
09
06
2019
pubmed:
27
6
2019
medline:
13
3
2020
entrez:
27
6
2019
Statut:
ppublish
Résumé
Banana cultivars are derived from hybridizations involving Musa acuminata subspecies. The latter diverged following geographical isolation in distinct South-east Asian continental regions and islands. Observation of chromosome pairing irregularities in meiosis of hybrids between these subspecies suggested the presence of large chromosomal structural variations. The aim of this study was to characterize such rearrangements. Marker (single nucleotide polymorphism) segregation in a self-progeny of the 'Calcutta 4' accession and mate-pair sequencing were used to search for chromosomal rearrangements in comparison with the M. acuminata ssp. malaccensis genome reference sequence. Signature segment junctions of the revealed chromosome structures were identified and searched in whole-genome sequencing data from 123 wild and cultivated Musa accessions. Two large reciprocal translocations were characterized in the seedy banana M. acuminata ssp. burmannicoides 'Calcutta 4' accession. One consisted of an exchange of a 240 kb distal region of chromosome 2 with a 7.2 Mb distal region of chromosome 8. The other involved an exchange of a 20.8 Mb distal region of chromosome 1 with a 11.6 Mb distal region of chromosome 9. Both translocations were found only in wild accessions belonging to the burmannicoides/burmannica/siamea subspecies. Only two of the 87 cultivars analysed displayed the 2/8 translocation, while none displayed the 1/9 translocation. Two large reciprocal translocations were identified that probably originated in the burmannica genetic group. Accurate characterization of these translocations should enhance the use of this disease resistance-rich burmannica group in breeding programmes.
Sections du résumé
BACKGROUND AND AIMS
Banana cultivars are derived from hybridizations involving Musa acuminata subspecies. The latter diverged following geographical isolation in distinct South-east Asian continental regions and islands. Observation of chromosome pairing irregularities in meiosis of hybrids between these subspecies suggested the presence of large chromosomal structural variations. The aim of this study was to characterize such rearrangements.
METHODS
Marker (single nucleotide polymorphism) segregation in a self-progeny of the 'Calcutta 4' accession and mate-pair sequencing were used to search for chromosomal rearrangements in comparison with the M. acuminata ssp. malaccensis genome reference sequence. Signature segment junctions of the revealed chromosome structures were identified and searched in whole-genome sequencing data from 123 wild and cultivated Musa accessions.
KEY RESULTS
Two large reciprocal translocations were characterized in the seedy banana M. acuminata ssp. burmannicoides 'Calcutta 4' accession. One consisted of an exchange of a 240 kb distal region of chromosome 2 with a 7.2 Mb distal region of chromosome 8. The other involved an exchange of a 20.8 Mb distal region of chromosome 1 with a 11.6 Mb distal region of chromosome 9. Both translocations were found only in wild accessions belonging to the burmannicoides/burmannica/siamea subspecies. Only two of the 87 cultivars analysed displayed the 2/8 translocation, while none displayed the 1/9 translocation.
CONCLUSION
Two large reciprocal translocations were identified that probably originated in the burmannica genetic group. Accurate characterization of these translocations should enhance the use of this disease resistance-rich burmannica group in breeding programmes.
Identifiants
pubmed: 31241133
pii: 5523264
doi: 10.1093/aob/mcz078
pmc: PMC6758587
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
319-329Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of the Annals of Botany Company.
Références
Mol Biol Evol. 2017 Sep 1;34(9):2140-2152
pubmed: 28575404
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
BMC Genomics. 2008 Jan 30;9:58
pubmed: 18234080
Theor Appl Genet. 1987 Apr;73(6):883-92
pubmed: 24241299
BMC Bioinformatics. 2015 Mar 06;16:73
pubmed: 25887893
Heredity (Edinb). 1948 Jun;2(Pt 1):101-17
pubmed: 18863987
BMC Plant Biol. 2010 Apr 13;10:65
pubmed: 20388207
Mol Biol Evol. 2019 Jan 1;36(1):97-111
pubmed: 30403808
Genome Res. 2010 Sep;20(9):1297-303
pubmed: 20644199
Genome Biol Evol. 2018 Dec 1;10(12):3129-3140
pubmed: 30321324
Genome Biol. 2016 Nov 25;17(1):239
pubmed: 27887629
Database (Oxford). 2013 May 23;2013:bat035
pubmed: 23707967
Nat Commun. 2018 Jul 6;9(1):2638
pubmed: 29980662
Theor Appl Genet. 1995 Dec;91(8):1195-202
pubmed: 24170046
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11311-8
pubmed: 21730145
BMC Genomics. 2016 Mar 16;17:243
pubmed: 26984673
Genome Res. 2009 Sep;19(9):1639-45
pubmed: 19541911
Genome Res. 2017 May;27(5):722-736
pubmed: 28298431
Ann Bot. 2016 Dec;118(7):1269-1278
pubmed: 27590334
Bioinformatics. 2011 Nov 1;27(21):2987-93
pubmed: 21903627
New Phytol. 2016 Jun;210(4):1453-65
pubmed: 26832306
Ann Bot. 2011 Oct;108(5):975-81
pubmed: 21835815
Nature. 2012 Aug 9;488(7410):213-7
pubmed: 22801500
Theor Appl Genet. 1993 Dec;87(4):517-26
pubmed: 24190325
Nat Commun. 2016 Apr 15;7:11307
pubmed: 27079541
Bioinformatics. 2007 Apr 15;23(8):1026-8
pubmed: 17309896
Database (Oxford). 2017 Jan 1;2017:
pubmed: 29220435
Nat Plants. 2018 Nov;4(11):879-887
pubmed: 30390080
BMC Bioinformatics. 2009 Dec 15;10:421
pubmed: 20003500