Proteomic analysis of excretory-secretory products from young adults of Angiostrongylus cantonensis.


Journal

Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
Titre abrégé: Mem Inst Oswaldo Cruz
Pays: Brazil
ID NLM: 7502619

Informations de publication

Date de publication:
2019
Historique:
received: 28 11 2018
accepted: 28 05 2019
entrez: 27 6 2019
pubmed: 27 6 2019
medline: 6 8 2019
Statut: ppublish

Résumé

Angiostrongyliasis is caused by the nematode Angiostrongylus cantonensis and can lead to eosinophilic meningitis and meningoencephalitis in humans. The young adult worms play central pathogenic roles in the central nervous system (CNS); however, the underlying mechanism is unclear. Excretory-secretory products (ESPs) are good investigation targets for studying the relationship between a host and its parasite. We aimed to profile, identify, and characterise the proteins in the ESPs of A. cantonensis young adults. The ESPs of young adult worms were collected from culture medium after incubation ranging from 24 to 96 h. Proteomic and bioinformatics analyses were performed to characterise the ESPs. A total of 51 spots were identified, and the highly expressed proteins included two protein disulphide isomerases, one calreticulin, and three uncharacterised proteins. Subsequently, approximately 254 proteins were identified in the ESPs of A. cantonensis young adults via liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and these were further classified according to their characteristics and biological functions. Finally, we identified the immunoreactive proteins from a reference map of ESPs from A. cantonensis young adults. Approximately eight proteins were identified, including a protein disulphide isomerase, a putative aspartic protease, annexin, and five uncharacterised proteins. The study established and identified protein reference maps for the ESPs of A. cantonensis young adults. The identified proteins may be potential targets for the development of diagnostic or therapeutic agents for human angiostrongyliasis.

Sections du résumé

BACKGROUND BACKGROUND
Angiostrongyliasis is caused by the nematode Angiostrongylus cantonensis and can lead to eosinophilic meningitis and meningoencephalitis in humans. The young adult worms play central pathogenic roles in the central nervous system (CNS); however, the underlying mechanism is unclear. Excretory-secretory products (ESPs) are good investigation targets for studying the relationship between a host and its parasite.
OBJECTIVES OBJECTIVE
We aimed to profile, identify, and characterise the proteins in the ESPs of A. cantonensis young adults.
METHODS METHODS
The ESPs of young adult worms were collected from culture medium after incubation ranging from 24 to 96 h. Proteomic and bioinformatics analyses were performed to characterise the ESPs.
FINDINGS RESULTS
A total of 51 spots were identified, and the highly expressed proteins included two protein disulphide isomerases, one calreticulin, and three uncharacterised proteins. Subsequently, approximately 254 proteins were identified in the ESPs of A. cantonensis young adults via liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and these were further classified according to their characteristics and biological functions. Finally, we identified the immunoreactive proteins from a reference map of ESPs from A. cantonensis young adults. Approximately eight proteins were identified, including a protein disulphide isomerase, a putative aspartic protease, annexin, and five uncharacterised proteins. The study established and identified protein reference maps for the ESPs of A. cantonensis young adults.
MAIN CONCLUSIONS CONCLUSIONS
The identified proteins may be potential targets for the development of diagnostic or therapeutic agents for human angiostrongyliasis.

Identifiants

pubmed: 31241649
pii: S0074-02762019000100334
doi: 10.1590/0074-02760180556
pmc: PMC6594673
pii:
doi:

Substances chimiques

Helminth Proteins 0

Types de publication

Journal Article

Langues

eng

Pagination

e180556

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Auteurs

Kuang-Yao Chen (KY)

China Medical University, School of Medicine, Department of Parasitology, Taichung, Taiwan.
Chang Gung University, College of Medicine, Department of Parasitology, Taoyuan, Taiwan.

Pei-Jhen Lu (PJ)

Chang Gung University, College of Medicine, Department of Parasitology, Taoyuan, Taiwan.

Chien-Ju Cheng (CJ)

Chang Gung University, College of Medicine, Department of Parasitology, Taoyuan, Taiwan.

Kai-Yuan Jhan (KY)

Chang Gung University, College of Medicine, Graduate Institute of Biomedical Sciences, Taoyuan, Taiwan.

Shih-Chien Yeh (SC)

Chang Gung University, College of Medicine, Department of Parasitology, Taoyuan, Taiwan.

Lian-Chen Wang (LC)

Chang Gung University, College of Medicine, Department of Parasitology, Taoyuan, Taiwan.
Chang Gung University, College of Medicine, Graduate Institute of Biomedical Sciences, Taoyuan, Taiwan.
Molecular Infectious Disease Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

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Classifications MeSH