Chronic stress induces hypersensitivity of murine gastric vagal afferents.
Afferent Pathways
/ physiology
Animals
Anxiety
/ etiology
Blood Glucose
/ analysis
Chronic Disease
Corticosterone
/ blood
Depression
/ etiology
Exploratory Behavior
Feeding Behavior
Gastric Emptying
Humans
Leptin
/ metabolism
Male
Maze Learning
Mechanoreceptors
/ physiology
Mice
Mice, Inbred C57BL
Noxae
Stress, Psychological
/ physiopathology
Sucrose
Swimming
Vagus Nerve
/ physiopathology
chronic stress
functional dyspepsia
gastric hypersensitivity
gastric vagal afferents
leptin
Journal
Neurogastroenterology and motility
ISSN: 1365-2982
Titre abrégé: Neurogastroenterol Motil
Pays: England
ID NLM: 9432572
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
20
02
2019
revised:
22
05
2019
accepted:
18
06
2019
pubmed:
27
6
2019
medline:
1
9
2020
entrez:
27
6
2019
Statut:
ppublish
Résumé
Stress exposure is known to trigger and exacerbate functional dyspepsia (FD) symptoms. Increased gastric sensitivity to food-related stimuli is widely observed in FD patients and is associated with stress and psychological disorders. The mechanisms underlying the hypersensitivity are not clear. Gastric vagal afferents (GVAs) play an important role in sensing meal-related mechanical stimulation to modulate gastrointestinal function and food intake. This study aimed to determine whether GVAs display hypersensitivity after chronic stress, and whether its interaction with leptin was altered by stress. Eight-week-old male C57BL/6 mice were exposed to unpredictable chronic mild stress or no stress (control) for 8 weeks. The metabolic rate, gastric emptying rate, and anxiety- and depression-like behaviors were determined. GVA mechanosensitivity, and its modulation by leptin, was determined using an in vitro single fiber recording technique. QRT-PCR was used to establish the levels of leptin and leptin receptor mRNA in the stomach and nodose ganglion, respectively. The stressed mice had lower body weight and food intake, and increased anxiety-like behavior compared to the control mice. The mechanosensitivity of mucosal and tension-sensitive GVAs was higher in the stressed mice. Leptin potentiated mucosal GVA mechanosensitivity in control but not stressed mice. The expression of leptin mRNA in the gastric mucosa was lower in the stressed mice. In conclusion, chronic stress enhances GVA mechanosensitivity, which may contribute to the gastric hypersensitivity in FD. In addition, the modulatory effect of leptin on GVA signaling is lost after chronic stress exposure.
Sections du résumé
BACKGROUND
Stress exposure is known to trigger and exacerbate functional dyspepsia (FD) symptoms. Increased gastric sensitivity to food-related stimuli is widely observed in FD patients and is associated with stress and psychological disorders. The mechanisms underlying the hypersensitivity are not clear. Gastric vagal afferents (GVAs) play an important role in sensing meal-related mechanical stimulation to modulate gastrointestinal function and food intake. This study aimed to determine whether GVAs display hypersensitivity after chronic stress, and whether its interaction with leptin was altered by stress.
METHODS
Eight-week-old male C57BL/6 mice were exposed to unpredictable chronic mild stress or no stress (control) for 8 weeks. The metabolic rate, gastric emptying rate, and anxiety- and depression-like behaviors were determined. GVA mechanosensitivity, and its modulation by leptin, was determined using an in vitro single fiber recording technique. QRT-PCR was used to establish the levels of leptin and leptin receptor mRNA in the stomach and nodose ganglion, respectively.
KEY RESULTS
The stressed mice had lower body weight and food intake, and increased anxiety-like behavior compared to the control mice. The mechanosensitivity of mucosal and tension-sensitive GVAs was higher in the stressed mice. Leptin potentiated mucosal GVA mechanosensitivity in control but not stressed mice. The expression of leptin mRNA in the gastric mucosa was lower in the stressed mice.
CONCLUSIONS AND INFERENCES
In conclusion, chronic stress enhances GVA mechanosensitivity, which may contribute to the gastric hypersensitivity in FD. In addition, the modulatory effect of leptin on GVA signaling is lost after chronic stress exposure.
Substances chimiques
Blood Glucose
0
Leptin
0
Noxae
0
Sucrose
57-50-1
Corticosterone
W980KJ009P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13669Informations de copyright
© 2019 John Wiley & Sons Ltd.
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