Prolidase enzyme is required for extracellular matrix integrity and impacts on postnatal cerebellar cortex development.
basement membrane
cerebellar development
cortical dysplasia
prolidase deficiency
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
01 01 2020
01 01 2020
Historique:
received:
23
01
2019
revised:
11
06
2019
accepted:
20
06
2019
pubmed:
28
6
2019
medline:
2
7
2021
entrez:
28
6
2019
Statut:
ppublish
Résumé
The extracellular matrix is essential for brain development, lamination, and synaptogenesis. In particular, the basement membrane below the pial meninx (pBM) is required for correct cortical development. The last step in the catabolism of the most abundant protein in pBM, collagen Type IV, requires prolidase, an exopeptidase cleaving the imidodipeptides containing pro or hyp at the C-terminal end. Mutations impairing prolidase activity lead in humans to the rare disease prolidase deficiency characterized by severe skin ulcers and mental impairment. Thus, the dark-like (dal) mouse, in which the prolidase is knocked-out, was used to investigate whether the deficiency of prolidase affects the neuronal maturation during development of a brain cortex area. Focusing on the cerebellar cortex, thinner collagen fibers and disorganized pBM were found. Aberrant cortical granule cell proliferation and migration occurred, associated to defects in brain lamination, and in particular in maturation of Purkinje neurons and formation of synaptic contacts. This study deeply elucidates a link between prolidase activity and neuronal maturation shedding new light on the molecular basis of functional aspects in the prolidase deficiency.
Substances chimiques
Dipeptidases
EC 3.4.13.-
proline dipeptidase
EC 3.4.13.9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-80Informations de copyright
© 2019 Wiley Periodicals, Inc.
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