Astrocyte lineage cells are essential for functional neuronal differentiation and synapse maturation in human iPSC-derived neural networks.
Action Potentials
/ physiology
Astrocytes
/ physiology
Cell Lineage
Cells, Cultured
Excitatory Postsynaptic Potentials
/ physiology
Glutamic Acid
/ metabolism
Humans
Induced Pluripotent Stem Cells
/ physiology
Miniature Postsynaptic Potentials
/ physiology
Neural Pathways
/ physiology
Neural Stem Cells
/ physiology
Neurogenesis
/ physiology
Neurons
/ physiology
Receptors, AMPA
/ metabolism
Synapses
/ physiology
human iPSCs
iPSC-derived astrocyte lineage cells
network function
neuronal differentiation
synapse maturation
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
15
05
2018
revised:
08
06
2019
accepted:
11
06
2019
pubmed:
28
6
2019
medline:
17
3
2020
entrez:
28
6
2019
Statut:
ppublish
Résumé
Human astrocytes differ dramatically in cell morphology and gene expression from murine astrocytes. The latter are well known to be of major importance in the formation of neuronal networks by promoting synapse maturation. However, whether human astrocyte lineage cells have a similar role in network formation has not been firmly established. Here, we investigated the impact of human astrocyte lineage cells on the functional maturation of neural networks that were derived from human induced pluripotent stem cells (hiPSCs). Initial in vitro differentiation of hiPSC-derived neural progenitor cells and immature neurons (glia+ cultures) resulted in spontaneously active neural networks as indicated by synchronous neuronal Ca
Substances chimiques
Receptors, AMPA
0
Glutamic Acid
3KX376GY7L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1893-1909Informations de copyright
© 2019 Wiley Periodicals, Inc.