Gold and silver bio/nano-hybrids-based electrochemical immunosensor for ultrasensitive detection of carcinoembryonic antigen.
Carcinoembryonic antigen
Electrochemical immunosensor
Streptavidin-coated gold NPs
Streptavidin-coated silver NPs
Thiolated graphene oxide
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Sep 2019
15 Sep 2019
Historique:
received:
06
04
2019
revised:
02
06
2019
accepted:
14
06
2019
pubmed:
28
6
2019
medline:
23
1
2020
entrez:
28
6
2019
Statut:
ppublish
Résumé
Carcinoembryonic antigen (CEA) is an important oncomarker for the detection of breast cancer. For ultra-sensitive sensing of CEA with great specificity and accuracy, an innovative and reliable electrochemical immunosensor was developed using various nano-hybrids. A glassy carbon electrode (GC) was modified with thiolated graphene oxide (T-GO) to elevate the active surface area of the electrode. The streptavidin-coated gold nanoparticles (AuNPs) were used to increase the conductivity of the sensing area as well as the loading capacity of the biotinylated monoclonal antibody (mAb). A sandwich-on approach was developed to reach a low limit of detection (LOD). The biotinylated mAb, streptavidin coated silver nanoparticles (AgNPs) and horseradish peroxidase (HRP), altogether, formed the signaling probe of the proposed immunosensor. The electrochemical signal was significantly enhanced in the presence of hydroquinone (HQ) and hydrogen peroxide (H
Identifiants
pubmed: 31247453
pii: S0956-5663(19)30518-4
doi: 10.1016/j.bios.2019.111439
pii:
doi:
Substances chimiques
Antibodies, Immobilized
0
Carcinoembryonic Antigen
0
graphene oxide
0
Silver
3M4G523W1G
Gold
7440-57-5
Graphite
7782-42-5
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111439Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.