High-resolution imaging reveals how the spindle midzone impacts chromosome movement.
Anaphase
Cell Cycle Proteins
/ metabolism
Chromosome Segregation
Chromosomes, Human
/ metabolism
Fluorescence Recovery After Photobleaching
HeLa Cells
Humans
Imaging, Three-Dimensional
Microtubule-Associated Proteins
/ metabolism
Microtubules
/ metabolism
Movement
Mutation
/ genetics
Spindle Apparatus
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 08 2019
05 08 2019
Historique:
received:
26
04
2019
revised:
21
05
2019
accepted:
30
05
2019
pubmed:
30
6
2019
medline:
19
5
2020
entrez:
29
6
2019
Statut:
ppublish
Résumé
In the spindle midzone, microtubules from opposite half-spindles form bundles between segregating chromosomes. Microtubule bundles can either push or restrict chromosome movement during anaphase in different cellular contexts, but how these activities are achieved remains poorly understood. Here, we use high-resolution live-cell imaging to analyze individual microtubule bundles, growing filaments, and chromosome movement in dividing human cells. Within bundles, filament overlap length marked by the cross-linking protein PRC1 decreases during anaphase as chromosome segregation slows. Filament ends within microtubule bundles appear capped despite dynamic PRC1 turnover and submicrometer proximity to growing microtubules. Chromosome segregation distance and rate are increased in two human cell lines when microtubule bundle assembly is prevented via PRC1 knockdown. Upon expressing a mutant PRC1 with reduced microtubule affinity, bundles assemble but chromosome hypersegregation is still observed. We propose that microtubule overlap length reduction, typically linked to pushing forces generated within filament bundles, is needed to properly restrict spindle elongation and position chromosomes within daughter cells.
Identifiants
pubmed: 31248912
pii: jcb.201904169
doi: 10.1083/jcb.201904169
pmc: PMC6683753
doi:
Substances chimiques
Cell Cycle Proteins
0
MAPRE1 protein, human
0
Microtubule-Associated Proteins
0
PRC1 protein, human
0
Banques de données
RefSeq
['NM_199413', 'NM_003981']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2529-2544Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM059363
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM065933
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130234
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130298
Pays : United States
Informations de copyright
© 2019 Pamula et al.
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