Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains.
beat pattern
cAMP
cilia
flagella
motility
navigation
optogenetics
sperm
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
27 06 2019
27 06 2019
Historique:
received:
21
05
2019
revised:
13
06
2019
accepted:
25
06
2019
entrez:
30
6
2019
pubmed:
30
6
2019
medline:
30
6
2019
Statut:
epublish
Résumé
Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3',5'-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To this end, we used sperm that express the light-activated phosphodiesterase LAPD or the photo-activated adenylate cyclase bPAC. The control of cAMP by LAPD or bPAC combined with pharmacological interventions provides spatiotemporal precision and allows to probe the physiological function of cAMP compartmentalization in mammalian sperm.
Identifiants
pubmed: 31252584
pii: cells8070648
doi: 10.3390/cells8070648
pmc: PMC6679001
pii:
doi:
Substances chimiques
Cyclic AMP
E0399OZS9N
Phosphoric Diester Hydrolases
EC 3.1.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
J Endocrinol Invest. 2000 Oct;23(9):578-83
pubmed: 11079452
Development. 2003 Apr;130(7):1317-26
pubmed: 12588848
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2993-8
pubmed: 14976244
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13483-8
pubmed: 15340140
Dev Cell. 2005 Aug;9(2):249-59
pubmed: 16054031
Biol Reprod. 2006 Jan;74(1):109-18
pubmed: 16177223
J Neurochem. 2006 Mar;96(6):1580-90
pubmed: 16464235
Dev Biol. 2006 Aug 15;296(2):353-62
pubmed: 16842770
Dev Biol. 2007 Dec 1;312(1):183-92
pubmed: 17950270
Asian J Androl. 2008 Jan;10(1):115-33
pubmed: 18087651
Hum Reprod Update. 2008 Nov-Dec;14(6):647-57
pubmed: 18653675
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20740-5
pubmed: 19074277
PLoS One. 2009 Dec 08;4(12):e8211
pubmed: 19997608
J Biol Chem. 2011 Jan 14;286(2):1181-8
pubmed: 21030594
EMBO J. 2012 Apr 4;31(7):1654-65
pubmed: 22354039
J Biol Chem. 2013 Dec 6;288(49):35307-20
pubmed: 24129574
Trends Cell Biol. 2014 Mar;24(3):198-207
pubmed: 24342435
Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8803-8
pubmed: 24889611
Elife. 2015 Jan 20;4:null
pubmed: 25601414
Syst Biol Reprod Med. 2015;61(6):345-52
pubmed: 26467841
Sci Rep. 2015 Nov 05;5:16146
pubmed: 26537127
Elife. 2016 Mar 22;5:
pubmed: 27003291
Development. 2016 Jul 1;143(13):2325-33
pubmed: 27226326
Cold Spring Harb Perspect Biol. 2017 Jul 5;9(7):null
pubmed: 28062561
Results Probl Cell Differ. 2017;59:241-267
pubmed: 28247052
Curr Opin Biotechnol. 2017 Dec;48:15-20
pubmed: 28288335
Mol Cell Endocrinol. 2018 Jun 15;468:111-120
pubmed: 29146556
Cells. 2018 Dec 26;8(1):
pubmed: 30587820
Development. 1995 Apr;121(4):1139-50
pubmed: 7538069
Biol Reprod. 1995 Jun;52(6):1227-33
pubmed: 7632830
Hum Reprod. 1998 May;13(5):1248-54
pubmed: 9647555