Interactions of Rabconnectin-3 with Cav2 calcium channels.
Cav2,2 calcium channels
Hippocampus
N-type channels
Opioid receptor
Rabconnectin-3
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
28 06 2019
28 06 2019
Historique:
received:
22
05
2019
accepted:
17
06
2019
entrez:
30
6
2019
pubmed:
30
6
2019
medline:
2
6
2020
Statut:
epublish
Résumé
This study describes the interaction between Cav2 calcium channels and Rabconnectin-3, a di-subunit protein that is associated with synaptic vesicles. Immunostaining reveals that both Rabconnectin-3α (RB-3α) and Rabconnectin-3β (RB-3β) are colocalized in mouse hippocampal neurons. Co-immunoprecipitations from brain tissue is consistent with the formation of a protein complex between RB-3α and RB-3β and both Cav2.2 and the related Cav2.1 calcium channel. The coexpression of either RB-3α or RB-3β with Cav2.2 calcium channels in tsA-201 cells led to a reduction in Cav2.2 current density without any effects on the voltage-dependence of activation or inactivation. Coexpression of both Rabconnectin-3 subunits did not cause an additive effect on current densities. Finally, the presence of Rabconnectin-3 did not interfere with μ-opioid receptor mediated Gβγ modulation of Cav2.2 channels. Altogether, our findings show that Rabconnectin-3 has the propensity to regulate calcium entry mediated by Cav2.2 channels.
Identifiants
pubmed: 31253182
doi: 10.1186/s13041-019-0483-y
pii: 10.1186/s13041-019-0483-y
pmc: PMC6599304
doi:
Substances chimiques
Cav2 protein, mouse
0
Caveolin 2
0
DMXL2 protein, mouse
0
Nerve Tissue Proteins
0
GTP-Binding Proteins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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