Actomyosin-driven force patterning controls endocytosis at the immune synapse.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 06 2019
28 06 2019
Historique:
received:
27
03
2019
accepted:
30
05
2019
entrez:
30
6
2019
pubmed:
30
6
2019
medline:
28
8
2019
Statut:
epublish
Résumé
An important channel of cell-to-cell communication is direct contact. The immune synapse is a paradigmatic example of such type of interaction: it forms upon engagement of antigen receptors in lymphocytes by antigen-presenting cells and allows the local exchange of molecules and information. Although mechanics has been shown to play an important role in this process, how forces organize and impact on synapse function is unknown. We find that mechanical forces are spatio-temporally patterned at the immune synapse: global pulsatile myosin II-driven tangential forces are observed at the synapse periphery while localised forces generated by invadosome-like F-actin protrusions are detected at its centre. Noticeably, we observe that these force-producing actin protrusions constitute the main site of antigen extraction and endocytosis and require myosin II contractility to form. The interplay between global and local forces dictated by the organization of the actomyosin cytoskeleton therefore controls endocytosis at the immune synapse.
Identifiants
pubmed: 31253773
doi: 10.1038/s41467-019-10751-7
pii: 10.1038/s41467-019-10751-7
pmc: PMC6599028
doi:
Substances chimiques
Receptors, Complement 3d
0
Actomyosin
9013-26-7
Cre recombinase
EC 2.7.7.-
Integrases
EC 2.7.7.-
Myosin Type II
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2870Subventions
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-10-JCJC-1504-Immuphy
Pays : International
Références
Sci Signal. 2018 Aug 07;11(542):
pubmed: 30087179
J Immunol. 2013 May 1;190(9):4661-75
pubmed: 23554309
Nature. 1988 Aug 25;334(6184):676-82
pubmed: 3261841
Cell. 2016 Jan 14;164(1-2):128-140
pubmed: 26771488
Biophys J. 2015 May 5;108(9):2181-90
pubmed: 25954876
Cell. 2013 May 23;153(5):948-62
pubmed: 23706734
J Cell Sci. 2016 Jul 15;129(14):2689-95
pubmed: 27401928
Mol Biol Cell. 2015 Apr 1;26(7):1273-85
pubmed: 25631815
Nat Rev Mol Cell Biol. 2006 Jun;7(6):404-14
pubmed: 16723976
Immunity. 2004 May;20(5):589-99
pubmed: 15142527
Eur J Immunol. 2015 Jun;45(6):1621-34
pubmed: 25756957
Cell. 2012 Dec 21;151(7):1513-27
pubmed: 23260139
Nat Rev Immunol. 2009 Jan;9(1):15-27
pubmed: 19079135
Nat Immunol. 2010 Oct;11(10):953-61
pubmed: 20835229
J Exp Med. 2009 Jul 6;206(7):1485-93
pubmed: 19506051
Nat Methods. 2010 Jul;7(7):560-6
pubmed: 20512144
Science. 2006 May 5;312(5774):738-41
pubmed: 16675699
Biophys J. 2008 Jan 1;94(1):207-20
pubmed: 17827246
Science. 2013 Jun 28;340(6140):1587-90
pubmed: 23686338
J Cell Biol. 2010 May 3;189(3):399-406
pubmed: 20439993
Cell Rep. 2018 May 22;23(8):2342-2353
pubmed: 29791846
J Immunol. 2012 Apr 15;188(8):3686-99
pubmed: 22442443
Nano Lett. 2016 Sep 14;16(9):5951-61
pubmed: 27559755
Biophys J. 2011 Aug 3;101(3):643-50
pubmed: 21806932
Elife. 2015 Aug 10;4:
pubmed: 26258882
J Cell Biol. 2015 Feb 2;208(3):259-71
pubmed: 25646084
Nat Methods. 2008 Aug;5(8):687-94
pubmed: 18604216
Science. 2017 May 12;356(6338):
pubmed: 28495700
Science. 2006 Jun 16;312(5780):1672-6
pubmed: 16778060
Nat Cell Biol. 2018 Mar;20(3):262-271
pubmed: 29403039
J Cell Biol. 2012 May 14;197(4):499-508
pubmed: 22564416
J Cell Biol. 2016 Nov 7;215(3):383-399
pubmed: 27799367
Curr Opin Immunol. 2001 Feb;13(1):26-31
pubmed: 11154913
Cell. 2012 Jun 8;149(6):1353-67
pubmed: 22682254
Nano Lett. 2016 Apr 13;16(4):2633-8
pubmed: 26923775
Nature. 2001 May 24;411(6836):489-94
pubmed: 11373683
Elife. 2018 Mar 19;7:
pubmed: 29553370
Immunol Rev. 2013 Jan;251(1):7-12
pubmed: 23278736
Mol Biol Cell. 2012 Aug;23(15):2891-904
pubmed: 22675027
EMBO J. 2015 Aug 13;34(16):2147-61
pubmed: 26124312
Sci Immunol. 2018 Nov 30;3(29):
pubmed: 30504208
Sci Immunol. 2017 Oct 27;2(16):
pubmed: 29079589
Immunity. 2013 Mar 21;38(3):461-74
pubmed: 23499492
J Cell Sci. 2016 May 1;129(9):1759-68
pubmed: 27029343
J Cell Biol. 2019 Apr 1;218(4):1138-1147
pubmed: 30659101
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):881-6
pubmed: 23277584
Biophys J. 1999 Apr;76(4):2307-16
pubmed: 10096925
Am J Physiol Cell Physiol. 2002 Mar;282(3):C595-605
pubmed: 11832345
Nat Methods. 2008 Jul;5(7):605-7
pubmed: 18536722
Biochem Biophys Res Commun. 2012 Oct 12;427(1):202-6
pubmed: 22995298
Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14825-30
pubmed: 21873247
Immunity. 2011 Sep 23;35(3):361-74
pubmed: 21820334
Nat Commun. 2014 Dec 10;5:5749
pubmed: 25494455
Methods Cell Biol. 2014;123:367-94
pubmed: 24974038