Fasciola hepatica in UK horses.
Fasciola hepatica
ELISA
horse
microsatellite
Journal
Equine veterinary journal
ISSN: 2042-3306
Titre abrégé: Equine Vet J
Pays: United States
ID NLM: 0173320
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
25
10
2018
revised:
14
04
2019
accepted:
15
06
2019
pubmed:
30
6
2019
medline:
2
7
2020
entrez:
30
6
2019
Statut:
ppublish
Résumé
Fasciola hepatica (liver fluke) affects grazing animals including horses but the extent to which it affects UK horses is unknown. To define how liver fluke affects the UK horse population. Descriptive, cross-sectional, observational study. An F. hepatica excretory-secretory antibody detection ELISA with a diagnostic sensitivity of 71% and specificity of 97% was validated and used to analyse serum samples. An abattoir study was performed to determine prevalence. A case-control study of 269 horses compared fluke exposure between horses with liver disease and controls. Data on clinical signs and blood test results were collected for sero-positive horses. Genotyping of adult fluke was used to produce a multilocus genotype for each parasite. Four (2.2%) of 183 horses registered in the UK, sampled in the abattoir, had adult flukes in the liver, and the sero-prevalence of F. hepatica was estimated as 8.7%. In the case-control study, horses showing signs consistent with liver disease had significantly higher odds of testing positive for F. hepatica on ELISA than control horses. In 23 sero-positive horses, a range of non-specific clinical signs and blood test abnormalities was reported, with a third of the horses showing no signs. Genotypic analysis of liver flukes from horses provided evidence that these came from the same population as flukes from sheep and cattle. Bias could have arisen in the prevalence and case-control studies due to convenience sampling methods, in particular the geographic origin of the horses. Only a small number of horses tested positive so the data on clinical signs are limited. Exposure to liver fluke occurs frequently in horses and may be an under-recognised cause of liver disease. Flukes isolated from horses are from the same population as those found in ruminants. When designing and implementing parasite control plans, fluke should be considered, and horses should be tested if appropriate.
Sections du résumé
BACKGROUND
BACKGROUND
Fasciola hepatica (liver fluke) affects grazing animals including horses but the extent to which it affects UK horses is unknown.
OBJECTIVES
OBJECTIVE
To define how liver fluke affects the UK horse population.
STUDY DESIGN
METHODS
Descriptive, cross-sectional, observational study.
METHODS
METHODS
An F. hepatica excretory-secretory antibody detection ELISA with a diagnostic sensitivity of 71% and specificity of 97% was validated and used to analyse serum samples. An abattoir study was performed to determine prevalence. A case-control study of 269 horses compared fluke exposure between horses with liver disease and controls. Data on clinical signs and blood test results were collected for sero-positive horses. Genotyping of adult fluke was used to produce a multilocus genotype for each parasite.
RESULTS
RESULTS
Four (2.2%) of 183 horses registered in the UK, sampled in the abattoir, had adult flukes in the liver, and the sero-prevalence of F. hepatica was estimated as 8.7%. In the case-control study, horses showing signs consistent with liver disease had significantly higher odds of testing positive for F. hepatica on ELISA than control horses. In 23 sero-positive horses, a range of non-specific clinical signs and blood test abnormalities was reported, with a third of the horses showing no signs. Genotypic analysis of liver flukes from horses provided evidence that these came from the same population as flukes from sheep and cattle.
MAIN LIMITATIONS
CONCLUSIONS
Bias could have arisen in the prevalence and case-control studies due to convenience sampling methods, in particular the geographic origin of the horses. Only a small number of horses tested positive so the data on clinical signs are limited.
CONCLUSIONS
CONCLUSIONS
Exposure to liver fluke occurs frequently in horses and may be an under-recognised cause of liver disease. Flukes isolated from horses are from the same population as those found in ruminants. When designing and implementing parasite control plans, fluke should be considered, and horses should be tested if appropriate.
Identifiants
pubmed: 31254486
doi: 10.1111/evj.13149
pmc: PMC7027485
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
194-199Subventions
Organisme : Animal Welfare Foundation (AWF)
Informations de copyright
© 2019 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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