Transient induction of Cdk9 in the early stage of differentiation is critical for myogenesis.
Cdk9
microRNA
myoblast C2C12
myogenic differentiation
stem cell
Journal
Journal of cellular biochemistry
ISSN: 1097-4644
Titre abrégé: J Cell Biochem
Pays: United States
ID NLM: 8205768
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
18
11
2018
revised:
24
05
2019
accepted:
29
05
2019
pubmed:
2
7
2019
medline:
9
9
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
Cdk9 is a serine-threonine protein kinase that has been recognized as a regulator of cardiac differentiation. Recently, we have reported that transient induction of Cdk9 using noncoding RNA targeting Cdk9 sequences results in efficient cardiac differentiation. Concerning Cdk9 regulatory roles, here, we proposed whether constant overexpression of Cdk9 might influence the differentiation of myoblast C2C12 cells into myotubes. We overexpressed Cdk9 in mouse myoblast C2C12 cells to investigate its regulatory roles on myogenic differentiation. Upon Cdk9 overexpression, the expression level of myogenic regulatory factors was determined. Moreover, the expression profile of three important myomiRs consist of miR 1, 133 and 206 was examined during the differentiation process. Although Cdk9 expression is necessary for inducing differentiation in the early stage of myogenesis, continuous Cdk9 expression inhibits differentiation by modulating myomiRs and myogenic gene expression. Our results indicate that the transient induction of Cdk9 in the early stage of differentiation is critical for myogenesis.
Substances chimiques
MicroRNAs
0
Cdk9 protein, mouse
EC 2.7.11.22
Cyclin-Dependent Kinase 9
EC 2.7.11.22
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18854-18861Informations de copyright
© 2019 Wiley Periodicals, Inc.