The pleiotropic effects of Innexin genes expressed in Drosophila glia encompass wing chemosensory sensilla.
Inx2
Drosophila wing
anterior wing margin nerve
calcium influx
chemosensilla
Journal
Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
04
03
2019
revised:
05
06
2019
accepted:
07
06
2019
pubmed:
2
7
2019
medline:
24
7
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
The neuroanatomy of Drosophila wing chemosensilla and the analysis of their sensory organ precursor cell lineage have demonstrated that they are surprisingly related to taste perception. The microarchitecture of wing bristles limits the use of electrophysiology methods to investigate wing chemosensory mechanisms. However, by monitoring the fluorescence of the complex calcium/GCaMP, calcium flux triggered upon tastant stimulation was observed within sensilla aligned along the wing anterior nerve. This string of fluorescent puncta was impaired in wings of Innexin 2 (Inx2) mutant flies; although it is unclear whether the Innexin proteins act at the level of the wing imaginal disc, adult wing and/or at both levels. Glial cells known to shelter Innexin(s) expression have no documented role in adult chemosensory sensilla. Our data suggest that Innexin(s) are likely required for the maturation of functional wing chemosensilla in adulthood. The unexpected presence of most Innexin transcripts in adult wing RNAseq data set argues for the expression of Innexin proteins in the larval imaginal wing disc that are continued in wing chemosensilla at adulthood. OPEN PRACTICES: This article has earned an Open Data badge for making publicly available the digitally-shareable data necessary to reproduce the reported results. The data is available as supporting materials and includes the electronic lab notebook. Learn more about the Open Practices badges from the Center for Open Science: https://osf.io/tvyxz/wiki.
Substances chimiques
Connexins
0
Drosophila Proteins
0
inx2 protein, Drosophila
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1319-1330Informations de copyright
© 2019 Wiley Periodicals, Inc.
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