Human Fetal Pigmented Ciliary Epithelium Stem Cells have Regenerative Capacity in the Murine Retinal Degeneration Model of Laser Injury.
Animals
Cells, Cultured
Cilia
/ physiology
Epithelial Cells
/ physiology
Fetal Stem Cells
/ chemistry
Humans
Lasers
/ adverse effects
Male
Mice
Mice, Inbred C57BL
Nerve Regeneration
/ physiology
Retinal Degeneration
/ etiology
Retinal Pigment Epithelium
/ physiology
Stem Cell Transplantation
/ methods
Age-related Macular Degeneration
(AMD)
Ciliary epithelium
laser injury
retinal degeneration
subretinal
transplantation.
Journal
Current neurovascular research
ISSN: 1875-5739
Titre abrégé: Curr Neurovasc Res
Pays: United Arab Emirates
ID NLM: 101208439
Informations de publication
Date de publication:
2019
2019
Historique:
received:
23
04
2019
revised:
29
04
2019
accepted:
30
04
2019
pubmed:
2
7
2019
medline:
1
9
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
Retinal degeneration and related eye disorders have limited treatment interventions. Since stem cell therapy has shown promising results, ciliary epithelium (CE) derived stem cells could be a better choice given the fact that cells from eye niche can better integrate with the degenerating retina, rewiring the synaptic damage. To test the effect of human fetal pigmented ciliary epithelium-derived neurospheres in the mouse model of laser-induced retinal degeneration. C57 male mice were subjected to retinal injury by Laser photocoagulation. Human fetal pigmented ciliary epithelium was obtained from post-aborted human eyeballs and cultured with epidermal growth factor (rhEGF) and fibroblast growth factor (rhFGF). The six day neurospheres were isolated, dissociated and transplanted into the subretinal space of the laser injured mice at the closest proximity to Laser shots. Mice were analyzed for functional vision through electroretinogram (ERG) and sacrificed at 1 week and 12 week time points. Retinal, Neurotropic, Apoptotic and proliferation markers were analysed using real-time polymerase chain reaction (PCR). The CE neurospheres showed an increase in the expression of candidate genes analyzed in the study at 1 week time point, which sustained for longer time point of 12 weeks. We showed the efficacy of human CE cells in the regeneration of retinal degeneration in murine model for the first time. CE cells need to be explored comprehensively both in disease and degeneration.
Sections du résumé
BACKGROUND
Retinal degeneration and related eye disorders have limited treatment interventions. Since stem cell therapy has shown promising results, ciliary epithelium (CE) derived stem cells could be a better choice given the fact that cells from eye niche can better integrate with the degenerating retina, rewiring the synaptic damage.
OBJECTIVE
To test the effect of human fetal pigmented ciliary epithelium-derived neurospheres in the mouse model of laser-induced retinal degeneration.
METHODS
C57 male mice were subjected to retinal injury by Laser photocoagulation. Human fetal pigmented ciliary epithelium was obtained from post-aborted human eyeballs and cultured with epidermal growth factor (rhEGF) and fibroblast growth factor (rhFGF). The six day neurospheres were isolated, dissociated and transplanted into the subretinal space of the laser injured mice at the closest proximity to Laser shots. Mice were analyzed for functional vision through electroretinogram (ERG) and sacrificed at 1 week and 12 week time points. Retinal, Neurotropic, Apoptotic and proliferation markers were analysed using real-time polymerase chain reaction (PCR).
RESULTS
The CE neurospheres showed an increase in the expression of candidate genes analyzed in the study at 1 week time point, which sustained for longer time point of 12 weeks.
CONCLUSION
We showed the efficacy of human CE cells in the regeneration of retinal degeneration in murine model for the first time. CE cells need to be explored comprehensively both in disease and degeneration.
Identifiants
pubmed: 31258084
pii: CNR-EPUB-98969
doi: 10.2174/1567202616666190618123931
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
187-193Informations de copyright
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