Humanized mice in cutaneous leishmaniasis-Suitability analysis of human PBMC transfer into immunodeficient mice.
Adoptive Transfer
/ adverse effects
Animals
Disease Progression
Graft vs Host Disease
/ etiology
Heterografts
Humans
Interferon-gamma
/ pharmacology
Leishmaniasis, Cutaneous
/ parasitology
Leukocytes, Mononuclear
/ transplantation
Macrophages
/ drug effects
Mice
Models, Animal
Species Specificity
T-Lymphocyte Subsets
/ immunology
Journal
Experimental dermatology
ISSN: 1600-0625
Titre abrégé: Exp Dermatol
Pays: Denmark
ID NLM: 9301549
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
08
03
2019
revised:
28
05
2019
accepted:
25
06
2019
pubmed:
2
7
2019
medline:
24
10
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
Humanized mice represent a suitable preclinical test system for example therapeutic interventions in various disease settings, including infections. Here, we intended to establish such system for cutaneous leishmaniasis by infecting T, B and NK cell-deficient mice adoptively transferred with human peripheral blood mononuclear cells (PBMC). L major infection led to the establishment of parasite lesions harbouring viable parasites and human T cells, but parasite elimination was not seen due to a species-specific activity of T cell-derived human IFNγ. In addition, up to 50% of infected mice succumbed to severe graft-versus-host disease. In summary, even though long-term disease outcome assessments are impossible, this model of humanized mice can be used for studying lesion development and generation of oligoclonal anti-parasite human T cell responses in vivo.
Substances chimiques
Interferon-gamma
82115-62-6
Types de publication
Letter
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1087-1090Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : STE 1208/11-1, 14-1, TR156, SFB 1292
Pays : International
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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