Dynactin pathway-related gene expression is altered by aging, but not by vitrification.
Aging
Aneuploidy
Cryopreservation
Dynactin
Gene expression
Oocyte
Journal
Reproductive toxicology (Elmsford, N.Y.)
ISSN: 1873-1708
Titre abrégé: Reprod Toxicol
Pays: United States
ID NLM: 8803591
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
26
02
2019
revised:
12
06
2019
accepted:
26
06
2019
pubmed:
2
7
2019
medline:
7
2
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility.
Identifiants
pubmed: 31260804
pii: S0890-6238(19)30088-7
doi: 10.1016/j.reprotox.2019.06.011
pii:
doi:
Substances chimiques
Dynactin Complex
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
48-55Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.