A validated LC-MS/MS method for the determination of homocysteic acid in biological samples.
Cerebrospinal fluid
HILIC
Homocysteic acid
SPE
Serum
Urine
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
10 Sep 2019
10 Sep 2019
Historique:
received:
12
03
2019
revised:
04
06
2019
accepted:
05
06
2019
pubmed:
2
7
2019
medline:
7
2
2020
entrez:
2
7
2019
Statut:
ppublish
Résumé
The role of homocysteic acid (HCA) in severe diseases like Alzheimer's disease is under discussion and some recent studies correlate elevated HCA concentrations with the diagnosis of Alzheimer's. However, non-selective and insufficiently sensitive methods have been used to quantitate HCA and results of different studies show large differences in the determined HCA concentration in samples from patients and controls, and therefore non-comparable results. An accurate and precise quantitation method for the determination of HCA in human serum, urine and CSF has been developed by using a combination of protein precipitation and solid phase extraction for sample preparation followed by an LC-MS/MS analysis using a combination of a HILIC separation and tandem mass spectrometry. The developed method has been fully validated in accordance with the guidelines provided by the US Food and Drug administration FDA and the European Medicines Agency EMA. Furthermore, the method has demonstrated its ability to determine the endogenous HCA concentration in serum and urine samples from healthy volunteers.
Identifiants
pubmed: 31261039
pii: S0731-7085(19)30626-0
doi: 10.1016/j.jpba.2019.06.008
pii:
doi:
Substances chimiques
Homocysteine
0LVT1QZ0BA
homocysteic acid
1001-13-4
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
578-587Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.