Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus).


Journal

Animal reproduction science
ISSN: 1873-2232
Titre abrégé: Anim Reprod Sci
Pays: Netherlands
ID NLM: 7807205

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 05 03 2019
revised: 23 05 2019
accepted: 11 06 2019
entrez: 4 7 2019
pubmed: 4 7 2019
medline: 18 1 2020
Statut: ppublish

Résumé

The development of analytical methods for the evaluation of crocodilian semen is an important component for the assessment of male breeding soundness and the development of assisted breeding technology in this taxon. Computer-Assisted Semen Analysis (CASA) technology is becoming an increasingly common technique in seminal evaluations for animals but there has been no application of this technique for reptilian spermatozoa. The aim of this study was to analyse sperm kinematic and morphometric variables in Caiman crocodilus fuscus semen samples and to determine whether there were sperm subpopulations. Four ejaculates from four sexually mature captive caimans were used for this study. A CASA-Mot and CASA-Morph system was used with an image acquisition rate of 50 Hz for 2 s of capture. The ISAS

Identifiants

pubmed: 31266600
pii: S0378-4320(19)30212-X
doi: 10.1016/j.anireprosci.2019.06.011
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

9-20

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Anthony Valverde (A)

Costa Rica Institute of Technology, School of Agronomy, San Carlos Campus, 223-21001, Alajuela, Costa Rica; University of Valencia, Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain. Electronic address: anvalverde@tec.ac.cr.

Mónica Madrigal-Valverde (M)

Costa Rica Institute of Technology, School of Agronomy, San Carlos Campus, 223-21001, Alajuela, Costa Rica; Federal University of Bahia, School of Veterinary Medicine and Animal Science, 40170-110, Salvador, Bahia, Brazil.

Olivier Castro-Morales (O)

Costa Rica Institute of Technology, School of Agronomy, San Carlos Campus, 223-21001, Alajuela, Costa Rica.

Arnoldo Gadea-Rivas (A)

Costa Rica Institute of Technology, School of Agronomy, San Carlos Campus, 223-21001, Alajuela, Costa Rica.

Stephen Johnston (S)

School of Agriculture and Food Science, The University of Queensland, Gatton, Qld, 4343, Australia.

Carles Soler (C)

University of Valencia, Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino, 9, Building 3 (CUE), Floor 1, 46980, Paterna, Valencia, Spain.

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Classifications MeSH