Measuring Sharp Waves and Oscillatory Population Activity With the Genetically Encoded Calcium Indicator GCaMP6f.
GCaMP
calcium signals
hippocampal sharp wave
hippocampal slice
local field potential recordings
theta oscillation
voltage-sensitive dye imaging
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2019
2019
Historique:
received:
04
02
2019
accepted:
05
06
2019
entrez:
6
7
2019
pubmed:
6
7
2019
medline:
6
7
2019
Statut:
epublish
Résumé
GCaMP6f is among the most widely used genetically encoded calcium indicators for monitoring neuronal activity. Applications are at both the cellular and population levels. Here, we explore two important and under-explored issues. First, we have tested if GCaMP6f is sensitive enough for the detection of population activity with sparse firing, similar to the sensitivity of the local field potential (LFP). Second, we have tested if GCaMP6f is fast enough for the detection of fast network oscillations critical for the encoding and consolidation of memory. We have focused this study on the activity of the hippocampal network including sharp waves (SWs), carbachol-induced theta oscillations, and interictal-like spikes. We compare simultaneous LFP and optical GCaMP6f fluorescent recordings in Thy1-GCaMP6f mouse hippocampal slices. We observe that SWs produce a clear population GCaMP6f signal above noise with an average magnitude of 0.3% Δ
Identifiants
pubmed: 31275115
doi: 10.3389/fncel.2019.00274
pmc: PMC6593119
doi:
Types de publication
Journal Article
Langues
eng
Pagination
274Subventions
Organisme : NIA NIH HHS
ID : F31 AG062030
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR001431
Pays : United States
Références
J Comp Neurol. 1978 Mar 1;178(1):73-88
pubmed: 75894
Sci Rep. 2015 Nov 03;5:15978
pubmed: 26527405
J Neurosci Methods. 2009 Oct 15;183(2):107-13
pubmed: 19524611
Curr Biol. 2013 Feb 18;23(4):307-11
pubmed: 23375894
Cell Calcium. 2005 Apr;37(4):283-99
pubmed: 15755490
Cell Rep. 2013 Sep 12;4(5):1010-21
pubmed: 23994479
Hippocampus. 2018 Jan;28(1):42-52
pubmed: 28921856
J Membr Biol. 1977 May 6;33(1-2):141-83
pubmed: 864685
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
J Neurosci. 1999 Jan 1;19(1):274-87
pubmed: 9870957
Nature. 1997 Jan 9;385(6612):161-5
pubmed: 8990119
PLoS One. 2009 Sep 07;4(9):e6925
pubmed: 19738897
Biophys J. 2004 May;86(5):3250-9
pubmed: 15111438
Neuron. 2012 Mar 8;73(5):862-85
pubmed: 22405199
Nature. 2013 Feb 14;494(7436):238-42
pubmed: 23354054
Nat Neurosci. 2005 Nov;8(11):1560-7
pubmed: 16222227
Hippocampus. 2015 Oct;25(10):1073-188
pubmed: 26135716
Adv Exp Med Biol. 2015;859:171-96
pubmed: 26238053
J Physiol. 2003 Aug 1;550(Pt 3):873-87
pubmed: 12807984
J Neurophysiol. 2003 Jan;89(1):81-9
pubmed: 12522161
J Comp Neurol. 1970 Apr;138(4):433-49
pubmed: 4907846
Cold Spring Harb Protoc. 2013 Oct 01;2013(10):918-24
pubmed: 24086061
PLoS One. 2014 Sep 24;9(9):e108697
pubmed: 25250714
Neuron. 2017 Oct 11;96(2):476-489.e5
pubmed: 29024667
Sci Rep. 2016 Dec 06;6:38276
pubmed: 27922063
Annu Rev Cell Dev Biol. 2000;16:521-55
pubmed: 11031246
J Neurosci. 2004 Nov 3;24(44):9897-902
pubmed: 15525774
J Comp Neurol. 1994 Jan 8;339(2):181-208
pubmed: 8300905
PLoS One. 2014 Aug 04;9(8):e104438
pubmed: 25089705
Cell Calcium. 2015 Dec;58(6):638-48
pubmed: 26572560
Brain Res. 2012 Jun 21;1461:1-9
pubmed: 22608077
J Neurosci. 2013 Jul 10;33(28):11677-91
pubmed: 23843535
Brain. 2014 Feb;137(Pt 2):463-85
pubmed: 24390441
J Physiol. 2015 May 15;593(10):2295-310
pubmed: 25781000
Science. 2018 Mar 30;359(6383):1524-1527
pubmed: 29439023
J Neurosci. 1999 Aug 15;19(16):RC20
pubmed: 10436076
Front Neurosci. 2018 Mar 15;12:164
pubmed: 29599704
Nat Methods. 2019 Jul;16(7):649-657
pubmed: 31209382
J Neurosci Methods. 2002 Mar 30;115(1):13-27
pubmed: 11897360
Brain Struct Funct. 2007 Jul;212(1):75-83
pubmed: 17717699
eNeuro. 2018 Feb 19;5(1):
pubmed: 29464198
J Neurosci. 1995 Jan;15(1 Pt 1):30-46
pubmed: 7823136
Cold Spring Harb Protoc. 2015 Feb 02;2015(2):155-67
pubmed: 25646507
J Physiol. 2004 Aug 1;558(Pt 3):953-61
pubmed: 15194734