Effects of short and long-term alcohol-based fixation on Sprague-Dawley rat tissue morphology, protein and nucleic acid preservation.

Animals Ethanol / chemistry Fixatives / chemistry Immunohistochemistry Organ Specificity Rats Rats, Sprague-Dawley Tissue Fixation
Alcohol DNA Fixative Formaldehyde Immunohistochemistry Pathology RNA

Journal

Acta histochemica
ISSN: 1618-0372
Titre abrégé: Acta Histochem
Pays: Germany
ID NLM: 0370320

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 11 05 2019
revised: 29 05 2019
accepted: 31 05 2019
pubmed: 7 7 2019
medline: 27 12 2019
entrez: 7 7 2019
Statut: ppublish

Résumé

Safety concerns on the toxic and carcinogenic effects of formalin exposure have drawn increasing attention to the search for alternative low risk fixatives for processing tissue specimens in laboratories worldwide. Alcohol-based fixatives are considered some of the most promising alternatives. We evaluated the performance of alcohol-fixed paraffin-embedded (AFPE) samples from Sprague-Dawley (SD) rats analyzing tissue morphology, protein and nucleic acid preservation after short and extremely long fixation times (up to 7 years), using formalin-fixed paraffin-embedded (FFPE) samples as a comparator fixative. Following short and long-term alcohol fixation, tissue morphology and cellular details in tissues, evaluated by scoring stained sections (Hematoxylin-Eosin and Mallory's trichrome), were optimally preserved if compared to formalin fixation. Immunoreactivity of proteins (Ki67, CD3, PAX5, CD68), evaluated by immunohistochemistry, showed satisfactory results when the fixation period did not exceed 1 year. Finally, we confirm the superiority of alcohol fixation compared to formalin, in terms of quantity of nucleic acid extracted from paraffin blocks, even after an extremely long time of alcohol fixation. Our results confirm that alcohol fixation is a suitable and safe alternative to formalin for pathological evaluations. There is a need for standardization of formalin-free methods and harmonization of diagnosis in pathology department worldwide.

Identifiants

DOI: 10.1016/j.acthis.2019.05.011 PMID: 31277893 PMC: PMC6939362
pubmed: 31277893
pii: S0065-1281(19)30163-1
doi: 10.1016/j.acthis.2019.05.011
pmc: PMC6939362
mid: NIHMS1546614
pii:
doi:

Substances chimiques

Fixatives 0
Ethanol 3K9958V90M

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

750-760

Subventions

Organisme : Intramural NIH HHS
ID : Z01 ES010017
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier GmbH. All rights reserved.

Références

Clin Neuropathol. 2016 Jan-Feb;35(1):3-12
pubmed: 26521938
Histopathology. 2012 Apr;60(5):804-15
pubmed: 22320393
Diagn Mol Pathol. 2006 Jun;15(2):115-23
pubmed: 16778593
Pathol Int. 1997 Oct;47(10):685-91
pubmed: 9361102
J Histochem Cytochem. 2011 Apr;59(4):366-81
pubmed: 21411808
PLoS One. 2011;6(11):e27704
pubmed: 22110732
Am J Pathol. 2002 Dec;161(6):1961-71
pubmed: 12466110
J Histochem Cytochem. 2018 Feb;66(2):121-135
pubmed: 29125916
J Mol Diagn. 2006 May;8(2):157-69
pubmed: 16645201
Exp Mol Pathol. 2006 Apr;80(2):183-91
pubmed: 16332367
BMC Clin Pathol. 2008 Jan 29;8:1
pubmed: 18230182
Ann Diagn Pathol. 2008 Dec;12(6):387-96
pubmed: 18995201
Pathol Res Pract. 1992 Oct;188(7):832-5
pubmed: 1448372
J Clin Pathol. 2010 Dec;63(12):1090-4
pubmed: 20978021
J Histochem Cytochem. 2007 Feb;55(2):105-9
pubmed: 16982846
Biotech Histochem. 2007 Jun;82(3):133-40
pubmed: 17987439
J Cell Biol. 1993 Nov;123(3):513-22
pubmed: 8227122
Toxicol Ind Health. 1989 Oct;5(5):699-730
pubmed: 2815102
Appl Immunohistochem Mol Morphol. 2008 Dec;16(6):513-20
pubmed: 18931614
Genes Dev. 1990 May;4(5):849-59
pubmed: 2116362
Neoplasma. 2003;50(4):300-4
pubmed: 12937845
Pathol Oncol Res. 2013 Jan;19(1):41-53
pubmed: 22893391
Pathol Res Pract. 2004;200(6):469-72
pubmed: 15310150
Eur J Clin Chem Clin Biochem. 1997 Aug;35(8):633-5
pubmed: 9298355
Am J Surg Pathol. 2014 Aug;38(8):1071-8
pubmed: 24705310
Arch Pathol Lab Med. 1994 Mar;118(3):298-302
pubmed: 8135636
Histol Histopathol. 2011 Jun;26(6):797-810
pubmed: 21472693
Appl Immunohistochem Mol Morphol. 2005 Sep;13(3):277-82
pubmed: 16082256
J Histochem Cytochem. 2010 Mar;58(3):237-46
pubmed: 19901271
J Proteome Res. 2010 Oct 1;9(10):5188-96
pubmed: 20812734
Environ Health Perspect. 2005 Sep;113(9):1205-8
pubmed: 16140628
Biotech Histochem. 2009 Oct;84(5):207-15
pubmed: 19886757
Am J Pathol. 2002 Feb;160(2):449-57
pubmed: 11839565
Methods. 2014 Nov;70(1):12-9
pubmed: 24561827
Hum Pathol. 2002 Jul;33(7):756-60
pubmed: 12196928
Appl Immunohistochem Mol Morphol. 2000 Sep;8(3):228-35
pubmed: 10981876
Acta Histochem. 2013 Apr;115(3):279-89
pubmed: 22921675
J Clin Pathol. 2013 Feb;66(2):124-35
pubmed: 23125305
Biotech Histochem. 2009 Oct;84(5):223-47
pubmed: 19886759
Lancet Oncol. 2009 Dec;10(12):1143-4
pubmed: 19998521
PLoS One. 2013;8(3):e60638
pubmed: 23555997
Environ Health. 2012 Sep 04;11:59
pubmed: 22947094
Anal Cell Pathol. 1997;15(2):119-29
pubmed: 9413596
J Histochem Cytochem. 2008 Nov;56(11):1033-42
pubmed: 18711211
Biotech Histochem. 2009 Oct;84(5):217-21
pubmed: 19886758
J Histochem Cytochem. 2016 Jul;64(7):425-40
pubmed: 27221702
Lab Invest. 2003 Oct;83(10):1427-35
pubmed: 14563944
Exp Mol Pathol. 2013 Feb;94(1):188-94
pubmed: 22814231
Ann N Y Acad Sci. 2002 Dec;982:87-105
pubmed: 12562630
Exp Mol Pathol. 2012 Feb;92(1):33-43
pubmed: 21963600
J Clin Pathol. 2011 Nov;64(11):960-7
pubmed: 21715573
Fed Regist. 1992 May 27;57(102):22290-328
pubmed: 10119140
Thyroid. 2009 Nov;19(11):1239-48
pubmed: 19888862
Am J Forensic Med Pathol. 2009 Sep;30(3):242-5
pubmed: 19696579
Diagn Mol Pathol. 2010 Jun;19(2):112-22
pubmed: 20502189
J Histochem Cytochem. 2009 Mar;57(3):257-64
pubmed: 19001637
Arch Pathol Lab Med. 2005 Apr;129(4):502-6
pubmed: 15794674
Appl Immunohistochem Mol Morphol. 2000 Mar;8(1):61-70
pubmed: 10937051
Toxicol Pathol. 2010 Jan;38(1):131-41
pubmed: 20028992
Toxicol Pathol. 2012;40(2):345-74
pubmed: 22434870
Exp Mol Pathol. 2011 Oct;91(2):490-5
pubmed: 21641900
Exp Mol Pathol. 2010 Apr;88(2):265-71
pubmed: 20025868
Appl Immunohistochem Mol Morphol. 2007 Jun;15(2):124-33
pubmed: 17525622
Genes Dev. 1992 Sep;6(9):1589-607
pubmed: 1516825
Am J Clin Pathol. 2011 Oct;136(4):548-56
pubmed: 21917676
Nucleic Acids Res. 2007;35(12):e85
pubmed: 17576663
Arch Pathol Lab Med. 2011 Jun;135(6):744-52
pubmed: 21631267

Auteurs

Simona Panzacchi (S)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Federica Gnudi (F)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Daniele Mandrioli (D)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Rita Montella (R)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Valentina Strollo (V)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Bruce Alexander Merrick (BA)

National Toxicology Program (NTP), National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park (RTP), NC, USA.

Fiorella Belpoggi (F)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy.

Eva Tibaldi (E)

Cesare Maltoni Cancer Research Center (CMCRC), Ramazzini Institute (RI), Bentivoglio, Bologna, Italy. Electronic address: tibaldie@ramazzini.it.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Éthanol › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Fixateurs › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Immunohistochimie › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Phénomènes physiologiques › Spécificité d'organe › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Effects of short and long-term alcohol-based...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Fixation tissulaire › Effects of short and long-term alcohol-based...