Degradation kinetics of α-conotoxin TxID.

Animals Conotoxins / chemical synthesis Conus Snail Hydrogen-Ion Concentration Kinetics Osmolar Concentration Protein Folding Temperature
UPLC activity assay stability and degradation kinetics α-Conotoxin TxID α3β4 nAChR

Journal

FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716

Informations de publication

Date de publication:
09 2019
Historique:
received: 15 04 2019
revised: 27 06 2019
accepted: 04 07 2019
pubmed: 7 7 2019
medline: 2 5 2020
entrez: 7 7 2019
Statut: ppublish

Résumé

α-Conotoxin (CTx) TxID is a potent α3β4 nicotinic acetylcholine receptor (nAChR) antagonist that has been suggested as a potential drug candidate to treat addiction and small cell lung cancer. The function and structure of TxID have been well-studied, but analyses of its stability have not previously been reported. The purpose of this study was to analyze the stability and forced degradation of TxID under various conditions: acid, alkali, water hydrolysis, oxidation, light, thiols, temperature, ionic strength and buffer pH. Different degradation products were formed under various conditions, and the degradation patterns of TxID showed pseudo-first-order kinetics. TxID degraded slowest at pH 3 within a pH range of 2-8. The major degradation products were analyzed using liquid chromatography-tandem mass spectrometry and the activity of the main product with α3β4 nAChR was analyzed using electrophysiological methods. Our analysis of TxID stability may aid the selection of appropriate conditions for peptide production, packaging and storage.

Identifiants

DOI: 10.1002/2211-5463.12697 PMID: 31278882 PMC: PMC6722883
pubmed: 31278882
doi: 10.1002/2211-5463.12697
pmc: PMC6722883
doi:

Substances chimiques

Conotoxins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1561-1572

Informations de copyright

© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

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Auteurs

Pan Xu (P)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Yang Xiong (Y)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Yiqiao Liu (Y)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Shurun Yu (S)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Dongting Zhangsun (D)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Yong Wu (Y)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.

Sulan Luo (S)

Key Laboratory of Tropical Biological Resources of Ministry of Education, Key Lab for Marine Drugs of Haikou, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan, China.
ORCID: 0000-0002-9358-5970

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Venins › Venins de mollusque › Conotoxines › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Mollusca › Gastropoda › Conus › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Phénomènes chimiques › Concentration osmolaire › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Degradation kinetics of α-conotoxin TxID.
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Degradation kinetics of α-conotoxin TxID.