Finasteride Enhances Stem Cell Signals of Human Dermal Papilla Cells.
Biomarkers
Cell Line
Cell Survival
/ drug effects
Cells, Cultured
Dermis
/ cytology
Finasteride
/ pharmacology
Fluorescent Antibody Technique
Hair Follicle
/ cytology
Humans
Phenotype
Proto-Oncogene Proteins c-akt
/ metabolism
Signal Transduction
/ drug effects
Stem Cells
/ cytology
Transcription Factors
/ metabolism
Wnt Signaling Pathway
/ drug effects
Finasteride
Protein kinase B
Wnt/β-catenin
dermal papilla
hair loss
stem cells
Journal
In vivo (Athens, Greece)
ISSN: 1791-7549
Titre abrégé: In Vivo
Pays: Greece
ID NLM: 8806809
Informations de publication
Date de publication:
Historique:
received:
29
04
2019
revised:
06
06
2019
accepted:
10
06
2019
entrez:
8
7
2019
pubmed:
8
7
2019
medline:
21
12
2019
Statut:
ppublish
Résumé
Finasteride (FN) has been widely used to treat androgenetic alopecia (AGA). This study aimed at exploring the effect of FN on DP stem cell properties. Effect of FN on stem cell properties was tested in a DP cell line and 2 human primary DP cells (HDPCs1 and HDPCs2). Cell toxicity and growth were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The aggregation behavior was observed by phase-contrast microscopy and a scanning electron microscope (SEM). Effects of FN on cell signaling were determined by western blotting and immunocytochemistry. Treatment of DPCs with FN was able to significantly increase their aggregation behavior and the expression of stem cell transcription factors Nanog and Sox-2, when compared to the non-treated control. FN up-regulated stem cell regulatory proteins through the activation of protein kinase B (AKT), β-catenin, and integrin-β1. FN had an interesting biological effect on stem cell induction. These findings support the use of this drug for hair loss control and the development of regeneration approaches.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Finasteride (FN) has been widely used to treat androgenetic alopecia (AGA). This study aimed at exploring the effect of FN on DP stem cell properties.
MATERIALS AND METHODS
METHODS
Effect of FN on stem cell properties was tested in a DP cell line and 2 human primary DP cells (HDPCs1 and HDPCs2). Cell toxicity and growth were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The aggregation behavior was observed by phase-contrast microscopy and a scanning electron microscope (SEM). Effects of FN on cell signaling were determined by western blotting and immunocytochemistry.
RESULTS
RESULTS
Treatment of DPCs with FN was able to significantly increase their aggregation behavior and the expression of stem cell transcription factors Nanog and Sox-2, when compared to the non-treated control. FN up-regulated stem cell regulatory proteins through the activation of protein kinase B (AKT), β-catenin, and integrin-β1.
CONCLUSION
CONCLUSIONS
FN had an interesting biological effect on stem cell induction. These findings support the use of this drug for hair loss control and the development of regeneration approaches.
Identifiants
pubmed: 31280211
pii: 33/4/1209
doi: 10.21873/invivo.11592
pmc: PMC6689352
doi:
Substances chimiques
Biomarkers
0
Transcription Factors
0
Finasteride
57GNO57U7G
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1209-1220Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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