Intracellular Signaling in Key Pathways Is Induced by Treatment with Ultrasound and Microbubbles in a Leukemia Cell Line, but Not in Healthy Peripheral Blood Mononuclear Cells.

cellular stress drug delivery intracellular signaling microbubbles phosphorylation sonoporation ultrasound ultrasound contrast agents

Journal

Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003

Informations de publication

Date de publication:
06 Jul 2019
Historique:
received: 05 06 2019
revised: 03 07 2019
accepted: 04 07 2019
entrez: 10 7 2019
pubmed: 10 7 2019
medline: 10 7 2019
Statut: epublish

Résumé

Treatment with ultrasound and microbubbles (sonoporation) to enhance therapeutic efficacy in cancer therapy is rapidly expanding, but there is still very little consensus as to why it works. Despite the original assumption that pore formation in the cell membrane is responsible for increased uptake of drugs, the molecular mechanisms behind this phenomenon are largely unknown. We treated cancer cells (MOLM-13) and healthy peripheral blood mononuclear cells (PBMCs) with ultrasound at three acoustic intensities (74, 501, 2079 mW/cm

Identifiants

pubmed: 31284599
pii: pharmaceutics11070319
doi: 10.3390/pharmaceutics11070319
pmc: PMC6680714
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Helse Vest
ID : 911182
Organisme : Helse Vest
ID : 912035
Organisme : National Institutes of Health
ID : R01CA199646
Organisme : Norges Forskningsråd
ID : 250317
Organisme : Kreftforeningen
ID : 182735
Organisme : Helse Vest
ID : 911779
Organisme : Kreftforeningen
ID : 6833652
Organisme : NCI NIH HHS
ID : R01 CA199646
Pays : United States

Références

Echocardiography. 1999 Oct;16(7, Pt 2):743-746
pubmed: 11175217
J Acoust Soc Am. 2001 Jul;110(1):588-96
pubmed: 11508983
J Acoust Soc Am. 2001 Jul;110(1):597-606
pubmed: 11508985
Ultrasound Med Biol. 2003 Aug;29(8):1211-22
pubmed: 12946524
Cytometry A. 2003 Oct;55(2):61-70
pubmed: 14505311
J Cell Mol Med. 2005 Jan-Mar;9(1):59-71
pubmed: 15784165
Mol Pharmacol. 2006 Jun;69(6):2047-57
pubmed: 16540596
Nat Methods. 2006 May;3(5):361-8
pubmed: 16628206
Biochim Biophys Acta. 2007 Aug;1773(8):1299-310
pubmed: 17188374
J Biol Chem. 2007 May 11;282(19):14056-64
pubmed: 17360704
Biochim Biophys Acta. 2007 Aug;1773(8):1358-75
pubmed: 17481747
Biochem Biophys Res Commun. 2007 Aug 10;359(4):860-5
pubmed: 17568561
Ultrasound Med Biol. 2008 May;34(5):824-33
pubmed: 18255220
Arthritis Res Ther. 2008;10(4):R77
pubmed: 18616830
Ultrasound Med Biol. 2009 May;35(5):847-60
pubmed: 19110370
Med Biol Eng Comput. 2009 Aug;47(8):893-900
pubmed: 19597745
Ultrason Sonochem. 2011 Sep;18(5):1218-24
pubmed: 21459032
J Invest Dermatol. 2012 Jul;132(7):1877-85
pubmed: 22418867
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Ultrasound Med Biol. 2012 Oct;38(10):1734-43
pubmed: 22920546
Endocr Relat Cancer. 2013 Mar 22;20(2):197-212
pubmed: 23329648
Ultrasound Med Biol. 2013 Jun;39(6):1075-86
pubmed: 23499345
Mol Pharm. 2013 Jul 1;10(7):2667-75
pubmed: 23675982
Mol Imaging Biol. 2014 Feb;16(1):53-62
pubmed: 23877869
Ultrasound Med Biol. 2013 Dec;39(12):2382-92
pubmed: 24063957
Adv Drug Deliv Rev. 2014 Jun;72:49-64
pubmed: 24270006
J Control Release. 2014 Jan 28;174:151-60
pubmed: 24291334
Biochem J. 2014 Mar 15;458(3):469-79
pubmed: 24438093
Ann Biomed Eng. 2014 Oct;42(10):2156-63
pubmed: 25096496
Biomed Tech (Berl). 2015 Feb;60(1):77-87
pubmed: 25367613
Cell Rep. 2015 Feb 10;10(5):684-693
pubmed: 25660019
Cancer Lett. 2015 May 1;360(2):160-70
pubmed: 25667120
Chemother Res Pract. 2015;2015:316015
pubmed: 26380110
Adv Exp Med Biol. 2016;880:263-91
pubmed: 26486343
Phys Biol. 2015 Nov 24;12(6):066007
pubmed: 26599283
J Control Release. 2016 Sep 28;238:157-165
pubmed: 27476609
Pharmacol Res. 2016 Nov;113(Pt A):216-227
pubmed: 27543462
J Control Release. 2016 Dec 10;243:172-181
pubmed: 27744037
J Control Release. 2017 Jan 10;245:70-80
pubmed: 27871988
J Control Release. 2018 Feb 28;272:169-181
pubmed: 29305924
Environ Health Perspect. 1997 Dec;105 Suppl 6:1575-8
pubmed: 9467085

Auteurs

Ragnhild Haugse (R)

Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway. ragnhild.haugse@uib.no.
Department of Quality and Development, Hospital Pharmacies Enterprise in Western Norway, Møllendalsbakken 9, 5021 Bergen, Norway. ragnhild.haugse@uib.no.
Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, Bergen 5021, Norway. ragnhild.haugse@uib.no.

Anika Langer (A)

Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway. Anika.Langer@uib.no.
Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, Bergen 5021, Norway. Anika.Langer@uib.no.

Stein-Erik Gullaksen (SE)

Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, Bergen 5021, Norway. stein.gullaksen@uib.no.
Department of Internal Medicine, Hematology Section, Haukeland University Hospital, Jonas Lies vei 65, 5021 Bergen, Norway. stein.gullaksen@uib.no.

Silje Maria Sundøy (SM)

Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway. silje_3006@hotmail.com.

Bjørn Tore Gjertsen (BT)

Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, Bergen 5021, Norway. Bjorn.Gjertsen@uib.no.
Department of Internal Medicine, Hematology Section, Haukeland University Hospital, Jonas Lies vei 65, 5021 Bergen, Norway. Bjorn.Gjertsen@uib.no.

Spiros Kotopoulis (S)

Phoenix Solutions AS, Ullernchausseen 64, 0379 Oslo, Norway. Spiros.Kotopoulis@uib.no.
Department of Clinical Medicine, The University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway. Spiros.Kotopoulis@uib.no.
National Centre for Ultrasound in Gastroenterology, Haukeland University Hospital, Jonas Lies vei 65, 5021 Bergen, Norway. Spiros.Kotopoulis@uib.no.

Emmet McCormack (E)

Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway. Emmet.Mc.Cormack@uib.no.
Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, The University of Bergen, Jonas Lies vei 65, Bergen 5021, Norway. Emmet.Mc.Cormack@uib.no.

Classifications MeSH