Melatonin is a potential drug for the prevention of bone loss during space flight.
Animals
Bone Density
/ drug effects
Bone Resorption
/ prevention & control
Calcitonin
/ metabolism
Cell Differentiation
/ drug effects
Goldfish
Immunohistochemistry
Melatonin
/ therapeutic use
NF-kappa B
/ metabolism
Osteoclasts
/ drug effects
RNA, Messenger
/ metabolism
Rats
Real-Time Polymerase Chain Reaction
Space Flight
Weightlessness
/ adverse effects
RANKL
calcitonin
fish scales
microgravity
osteoblasts
osteoclasts
Journal
Journal of pineal research
ISSN: 1600-079X
Titre abrégé: J Pineal Res
Pays: England
ID NLM: 8504412
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
20
02
2019
revised:
19
06
2019
accepted:
19
06
2019
pubmed:
10
7
2019
medline:
24
3
2020
entrez:
10
7
2019
Statut:
ppublish
Résumé
Astronauts experience osteoporosis-like loss of bone mass because of microgravity conditions during space flight. To prevent bone loss, they need a riskless and antiresorptive drug. Melatonin is reported to suppress osteoclast function. However, no studies have examined the effects of melatonin on bone metabolism under microgravity conditions. We used goldfish scales as a bone model of coexisting osteoclasts and osteoblasts and demonstrated that mRNA expression level of acetylserotonin O-methyltransferase, an enzyme essential for melatonin synthesis, decreased significantly under microgravity. During space flight, microgravity stimulated osteoclastic activity and significantly increased gene expression for osteoclast differentiation and activation. Melatonin treatment significantly stimulated Calcitonin (an osteoclast-inhibiting hormone) mRNA expression and decreased the mRNA expression of receptor activator of nuclear factor κB ligand (a promoter of osteoclastogenesis), which coincided with suppressed gene expression levels for osteoclast functions. This is the first study to report the inhibitory effect of melatonin on osteoclastic activation by microgravity. We also observed a novel action pathway of melatonin on osteoclasts via an increase in CALCITONIN secretion. Melatonin could be the source of a potential novel drug to prevent bone loss during space flight.
Identifiants
pubmed: 31286565
doi: 10.1111/jpi.12594
pmc: PMC6771646
doi:
Substances chimiques
NF-kappa B
0
RNA, Messenger
0
Calcitonin
9007-12-9
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12594Subventions
Organisme : Grant-in-Aid for Scientific Research [C] by JSPS
ID : 24620004, 18K11016
Organisme : Grants of Japan Aerospace Exploration Agency
Organisme : The cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University
ID : 17011
Organisme : Kanazawa University CHOZEN project.
Informations de copyright
© 2019 The Authors. Journal of Pineal Research Published by John Wiley & Sons Ltd.
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