Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans.
Animals
Antioxidants
/ pharmacology
Body Size
/ drug effects
Caenorhabditis elegans
/ drug effects
Caenorhabditis elegans Proteins
/ metabolism
Caesalpinia
/ chemistry
Flavonoids
/ analysis
Free Radical Scavengers
/ pharmacology
Longevity
/ drug effects
Methanol
Naphthoquinones
Phenols
/ analysis
Plant Extracts
/ pharmacology
Plant Leaves
/ chemistry
Reactive Oxygen Species
Reproduction
/ drug effects
Aging
Antioxidant
Caenorhabditis elegans
Caesalpinia mimosoides
DAF-16
Oxidative stress
Journal
BMC complementary and alternative medicine
ISSN: 1472-6882
Titre abrégé: BMC Complement Altern Med
Pays: England
ID NLM: 101088661
Informations de publication
Date de publication:
08 Jul 2019
08 Jul 2019
Historique:
received:
15
04
2019
accepted:
26
06
2019
entrez:
10
7
2019
pubmed:
10
7
2019
medline:
4
12
2019
Statut:
epublish
Résumé
Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context. C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated. A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction. The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
Sections du résumé
BACKGROUND
BACKGROUND
Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.
METHODS
METHODS
C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.
RESULTS
RESULTS
A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.
CONCLUSIONS
CONCLUSIONS
The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.
Identifiants
pubmed: 31286949
doi: 10.1186/s12906-019-2578-5
pii: 10.1186/s12906-019-2578-5
pmc: PMC6615182
doi:
Substances chimiques
Antioxidants
0
Caenorhabditis elegans Proteins
0
Flavonoids
0
Free Radical Scavengers
0
Naphthoquinones
0
Phenols
0
Plant Extracts
0
Reactive Oxygen Species
0
juglone
W6Q80SK9L6
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
164Subventions
Organisme : Chulalongkorn University
ID : GCUGR1125612058D
Références
Asian Pac J Cancer Prev. 2016;17(3):1341-5
pubmed: 27039769
Nature. 2000 Nov 9;408(6809):239-47
pubmed: 11089981
Mol Cell Biol. 2009 May;29(10):2704-15
pubmed: 19273594
Medicines (Basel). 2017 Aug 15;4(3):
pubmed: 28930275
BMC Complement Altern Med. 2015 Aug 18;15:285
pubmed: 26282848
Mech Ageing Dev. 1986 Jun;35(1):79-94
pubmed: 3736133
Oxid Med Cell Longev. 2019 Jun 2;2019:9012396
pubmed: 31281595
Planta Med. 2009 Feb;75(3):216-21
pubmed: 19085685
Clin Interv Aging. 2018 Apr 26;13:757-772
pubmed: 29731617
Chem Res Toxicol. 2004 Jan;17(1):55-62
pubmed: 14727919
Food Chem Toxicol. 2018 Oct;120:639-650
pubmed: 30077708
Food Funct. 2016 Feb;7(2):943-52
pubmed: 26726147
BMC Complement Altern Med. 2017 Dec 28;17(1):551
pubmed: 29282044
J Pharm Pharmacol. 2013 May;65(5):682-8
pubmed: 23600385
Antioxidants (Basel). 2014 Mar 04;3(1):129-43
pubmed: 26784668
Front Physiol. 2018 May 17;9:477
pubmed: 29867535
Phytomedicine. 2014 Mar 15;21(4):547-50
pubmed: 24252337
Methods Mol Biol. 2010;594:57-72
pubmed: 20072909
J Agric Food Chem. 2013 Mar 27;61(12):3047-53
pubmed: 23470220
Molecules. 2018 Feb 27;23(3):
pubmed: 29495517
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D411-7
pubmed: 14681445
BMC Complement Altern Med. 2018 Oct 16;18(1):278
pubmed: 30326896
PeerJ. 2016 Apr 07;4:e1879
pubmed: 27077003
Aging (Albany NY). 2009 Jun 15;1(6):573-7
pubmed: 20157538
Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):314-23
pubmed: 18024103
Int J Biol Macromol. 2016 Nov;92:842-849
pubmed: 27492557
PeerJ. 2018 Jul 13;6:e5159
pubmed: 30023139
Science. 1998 Dec 11;282(5396):2012-8
pubmed: 9851916
Aging Cell. 2003 Apr;2(2):111-21
pubmed: 12882324
Genome Res. 2005 Dec;15(12):1651-60
pubmed: 16339362
Biol Pharm Bull. 2007 Jun;30(6):1052-5
pubmed: 17541153
Biochem J. 2008 Jan 1;409(1):205-13
pubmed: 17714076
Nutrition. 2002 Oct;18(10):872-9
pubmed: 12361782
J Agric Food Chem. 2016 Feb 17;64(6):1283-90
pubmed: 26809379
Ann N Y Acad Sci. 1999;893:13-8
pubmed: 10672226
Phytochemistry. 2010 Oct;71(14-15):1756-64
pubmed: 20656305
Trends Ecol Evol. 2006 Jun;21(6):334-40
pubmed: 16769434
BMC Complement Altern Med. 2018 Jul 24;18(1):223
pubmed: 30041641
Interdiscip Top Gerontol. 2014;39:86-107
pubmed: 24862016
Physiol Rev. 1994 Jan;74(1):139-62
pubmed: 8295932
FASEB J. 2003 Dec;17(15):2305-7
pubmed: 14525938
Exp Gerontol. 2006 Oct;41(10):928-34
pubmed: 16839734
Adv Exp Med Biol. 1989;266:17-26; discussion 27-9
pubmed: 2486150
Asian Pac J Cancer Prev. 2014;15(1):449-54
pubmed: 24528072