The optoelectronic microrobot: A versatile toolbox for micromanipulation.
dielectrophoresis
microrobotics
optoelectronic tweezers
single-cell RNA sequencing
single-cell manipulation
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
23 07 2019
23 07 2019
Historique:
pubmed:
11
7
2019
medline:
1
4
2020
entrez:
11
7
2019
Statut:
ppublish
Résumé
Microrobotics extends the reach of human-controlled machines to submillimeter dimensions. We introduce a microrobot that relies on optoelectronic tweezers (OET) that is straightforward to manufacture, can take nearly any desirable shape or form, and can be programmed to carry out sophisticated, multiaxis operations. One particularly useful program is a serial combination of "load," "transport," and "deliver," which can be applied to manipulate a wide range of micrometer-dimension payloads. Importantly, microrobots programmed in this manner are much gentler on fragile mammalian cells than conventional OET techniques. The microrobotic system described here was demonstrated to be useful for single-cell isolation, clonal expansion, RNA sequencing, manipulation within enclosed systems, controlling cell-cell interactions, and isolating precious microtissues from heterogeneous mixtures. We propose that the optoelectronic microrobotic system, which can be implemented using a microscope and consumer-grade optical projector, will be useful for a wide range of applications in the life sciences and beyond.
Identifiants
pubmed: 31289234
pii: 1903406116
doi: 10.1073/pnas.1903406116
pmc: PMC6660717
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14823-14828Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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