Bone Marrow Immunohistochemistry and Flow Cytometry in the Diagnosis of Malignant Hematologic Diseases With Emphasis on Lymphomas: A Comparative Retrospective Study.
Biopsy
Bone Marrow
/ pathology
Flow Cytometry
/ methods
Hematologic Neoplasms
/ diagnosis
Humans
Immunohistochemistry
/ methods
Immunophenotyping
Leukemia
/ diagnosis
Leukemia, Myeloid, Acute
/ diagnosis
Leukemia, T-Cell
/ diagnosis
Lymphoma
/ diagnosis
Lymphoma, B-Cell
/ diagnosis
Myelodysplastic Syndromes
/ diagnosis
Retrospective Studies
Journal
Applied immunohistochemistry & molecular morphology : AIMM
ISSN: 1533-4058
Titre abrégé: Appl Immunohistochem Mol Morphol
Pays: United States
ID NLM: 100888796
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
pubmed:
11
7
2019
medline:
27
7
2021
entrez:
11
7
2019
Statut:
ppublish
Résumé
We aim to evaluate the degree of agreement between immunohistochemistry (IHC) and flow cytometry (FC) in the diagnosis of malignant hematologic diseases, mainly lymphomas. A total of 260 bone marrow biopsies, 255 bone marrow aspirates, and 5 other suspensions of 260 patients used for diagnosis of a hematologic malignancy between 2009 and 2012 with both, IHC and FC, were retrospectively analyzed. Overall there is a substantial degree of agreement (κ=0.69) between IHC and FC. Chronic lymphocytic leukemia/small lymphocytic lymphoma, mature T-cell neoplasms, acute leukemias, and myelodysplastic syndromes had the highest concurrence rates (>80%). In nonconcordant cases, an IHC provided diagnosis in 25.4%, and an FC in 4.6%. Lymphomas were diagnosed by an IHC only in 51% of the cases. Both methods have good concurrence rates and are complementary. An IHC has the advantage of combining markers, morphology, and tissue immunoarchitecture, which is beneficial in the diagnosis of lymphomas. An FC is required in leukemias as it is faster and plays an important role in minimal residual disease.
Identifiants
pubmed: 31290784
doi: 10.1097/PAI.0000000000000787
pii: 00129039-202008000-00004
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
508-512Références
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