Cutting Edge: Protein Arginine Deiminase 2 and 4 Regulate NLRP3 Inflammasome-Dependent IL-1β Maturation and ASC Speck Formation in Macrophages.


Journal

Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R

Informations de publication

Date de publication:
15 08 2019
Historique:
received: 30 05 2018
accepted: 15 06 2019
pubmed: 12 7 2019
medline: 10 4 2020
entrez: 12 7 2019
Statut: ppublish

Résumé

Protein arginine deiminase (PAD) enzymes catalyze the conversion of protein-bound arginine into citrulline, an irreversible posttranslational modification with loss of a positive charge that can influence protein-protein interactions and protein structure. Protein arginine deiminase activity depends on high intracellular calcium concentrations occurring in dying cells. In this study, we demonstrate that protein citrullination is common during pyroptotic cell death in macrophages and that inhibition of PAD enzyme activity by Cl-amidine, a pan-PAD inhibitor, blocks NLRP3 inflammasome assembly and proinflammatory IL-1β release in macrophages. Genetic deficiency of either PAD2 or PAD4 alone in murine macrophages does not impair IL-1β release; however, pharmacological inhibition or small interfering RNA knockdown of PAD2 within PAD4

Identifiants

pubmed: 31292215
pii: jimmunol.1800720
doi: 10.4049/jimmunol.1800720
pmc: PMC6690178
mid: NIHMS1044765
doi:

Substances chimiques

CARD Signaling Adaptor Proteins 0
Inflammasomes 0
Interleukin-1beta 0
NLR Family, Pyrin Domain-Containing 3 Protein 0
Protein-Arginine Deiminases EC 3.5.3.15

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

795-800

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM118112
Pays : United States

Informations de copyright

Copyright © 2019 by The American Association of Immunologists, Inc.

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Auteurs

Neha Mishra (N)

Section of Rheumatology, Department of Medicine A, University Medicine Greifswald, 17475 Greifswald, Germany.

Lidja Schwerdtner (L)

Section of Rheumatology, Department of Medicine A, University Medicine Greifswald, 17475 Greifswald, Germany.

Kelly Sams (K)

Department of Biomedical Sciences, Baker Institute for Animal Health, Cornell University, Ithaca, NY 14853.

Santanu Mondal (S)

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.

Fareed Ahmad (F)

Department of Dermatology, University of Heidelberg, 69120 Heidelberg, Germany.

Reinhold E Schmidt (RE)

Department of Clinical Immunology and Rheumatology, Hannover Medical School, 30625 Hannover, Germany; and.

Scott A Coonrod (SA)

Department of Biomedical Sciences, Baker Institute for Animal Health, Cornell University, Ithaca, NY 14853.

Paul R Thompson (PR)

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.

Markus M Lerch (MM)

Department of Medicine A, University Medicine Greifswald, 1745 Greifswald, Germany.

Lukas Bossaller (L)

Section of Rheumatology, Department of Medicine A, University Medicine Greifswald, 17475 Greifswald, Germany; lukas.bossaller@med.uni-greifswald.de.

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Classifications MeSH