Effect of Spermatic Nuclear Quality on Live Birth Rates in Intracytoplasmic Sperm Injection.
Intracytoplasmic sperm injection
live birth rate
sperm DNA fragmentation
sperm chromatin condensation
Journal
Journal of human reproductive sciences
ISSN: 0974-1208
Titre abrégé: J Hum Reprod Sci
Pays: India
ID NLM: 101473512
Informations de publication
Date de publication:
Historique:
entrez:
12
7
2019
pubmed:
12
7
2019
medline:
12
7
2019
Statut:
ppublish
Résumé
Our study defines the clinical role of sperm DNA damage in the assisted reproductive technology procedure. To investigate if the compaction of chromatin explored added to the analysis of the sperm DNA fragmentation allows obtaining a new indicator for sperm genome quality linked to live birth rate (LBR). This was a prospective study, undergoing 101 cycles in the intracytoplasmic sperm injection (ICSI) program. The sperm DNA fragmentation index (DFI) has been measured with sperm chromatin dispersion examination. The sperm decondensation index (SDI) of chromatin has been measured with aniline blue procedure; with these indexes, a new parameter has been created: DFI × SDI. Pearson's correlation coefficient, Student's The sperm concentration, motility, and normal morphology were lower when the DFI was high ( The DFI is a good prognostic for a delivery rate in ICSI procedure, and the SDI could be added to DFI to create a new parameter of sperm nuclear quality. This new parameter seems to be linked to LBR.
Sections du résumé
BACKGROUND
BACKGROUND
Our study defines the clinical role of sperm DNA damage in the assisted reproductive technology procedure.
AIM
OBJECTIVE
To investigate if the compaction of chromatin explored added to the analysis of the sperm DNA fragmentation allows obtaining a new indicator for sperm genome quality linked to live birth rate (LBR).
DESIGN
METHODS
This was a prospective study, undergoing 101 cycles in the intracytoplasmic sperm injection (ICSI) program.
MATERIALS AND METHODS
METHODS
The sperm DNA fragmentation index (DFI) has been measured with sperm chromatin dispersion examination. The sperm decondensation index (SDI) of chromatin has been measured with aniline blue procedure; with these indexes, a new parameter has been created: DFI × SDI.
STATISTICAL ANALYSIS
METHODS
Pearson's correlation coefficient, Student's
RESULTS
RESULTS
The sperm concentration, motility, and normal morphology were lower when the DFI was high (
CONCLUSION
CONCLUSIONS
The DFI is a good prognostic for a delivery rate in ICSI procedure, and the SDI could be added to DFI to create a new parameter of sperm nuclear quality. This new parameter seems to be linked to LBR.
Identifiants
pubmed: 31293326
doi: 10.4103/jhrs.JHRS_81_18
pii: JHRS-12-122
pmc: PMC6594123
doi:
Types de publication
Journal Article
Langues
eng
Pagination
122-129Déclaration de conflit d'intérêts
There are no conflicts of interest.
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