Analyses of rRNA gene chromatin in cell cycle arrested
Alpha-factor
Chromatin
Hydroxyurea
Psoralen crosslinking
RNA polymerase I
rRNA genes
Journal
Data in brief
ISSN: 2352-3409
Titre abrégé: Data Brief
Pays: Netherlands
ID NLM: 101654995
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
24
04
2019
revised:
17
05
2019
accepted:
23
05
2019
entrez:
12
7
2019
pubmed:
12
7
2019
medline:
12
7
2019
Statut:
epublish
Résumé
The existence of two chromatin structures in the rDNA locus was previously demonstrated for a large variety of organisms, ranging from yeast to human. In yeast there are about 150-200 rRNA genes organized in tandem repeats. Almost half of them are transcribed and largely depleted of nucleosomes (active/open), the other half is not transcribed and is assembled in regular arrays of nucleosomes (inactive/closed). It is proposed that RNA polymerase-I (RNAPI) transcription-elongation removes nucleosomes from closed rRNA genes (opening), and that soon after DNA replication there is deposition of nucleosomes on the open rRNA genes (closing). In G1 arrested cells, nearly all rRNA genes are depleted of nucleosomes, but most of them are not transcribed (inactive/open). In relation to the research article by
Identifiants
pubmed: 31294051
doi: 10.1016/j.dib.2019.104083
pii: S2352-3409(19)30437-8
pii: 104083
pmc: PMC6595284
doi:
Types de publication
Journal Article
Langues
eng
Pagination
104083Subventions
Organisme : NIEHS NIH HHS
ID : R01 ES002614
Pays : United States
Organisme : NIEHS NIH HHS
ID : R37 ES002614
Pays : United States
Références
Mol Cell Biol. 1999 Apr;19(4):3184-97
pubmed: 10082585
Peptides. 2004 Sep;25(9):1465-76
pubmed: 15374648
Biochem Cell Biol. 2005 Aug;83(4):449-59
pubmed: 16094448
Mutat Res. 2006 Jul 14;606(1-2):92-105
pubmed: 16713735
Nat Protoc. 2006;1(4):1922-8
pubmed: 17487177
Mutat Res. 2010 Apr 30;698(1-2):52-9
pubmed: 20348017
Curr Opin Hematol. 2011 May;18(3):158-65
pubmed: 21372708
Pediatr Dent. 2011 Jan-Feb;33(1):67-74
pubmed: 21406151
Cell. 2011 May 13;145(4):543-54
pubmed: 21565613
Nucleic Acids Res. 2014 Jan;42(1):380-95
pubmed: 24097442
Cell. 1989 Jun 2;57(5):753-61
pubmed: 2720786
Mutat Res. 2019 May;815:20-29
pubmed: 31063901