Season and size of urban particulate matter differentially affect cytotoxicity and human immune responses to Mycobacterium tuberculosis.
Adolescent
Adult
Aged
Air Pollutants
/ toxicity
Cities
Cytotoxicity, Immunologic
/ drug effects
Environmental Exposure
/ adverse effects
Female
Host Microbial Interactions
/ drug effects
Humans
In Vitro Techniques
Interferon-gamma
/ biosynthesis
Interleukin-1beta
/ biosynthesis
Leukocytes, Mononuclear
/ drug effects
Male
Mexico
Middle Aged
Mycobacterium tuberculosis
/ growth & development
Particle Size
Particulate Matter
/ toxicity
Phagocytosis
/ drug effects
Seasons
T-Box Domain Proteins
/ immunology
Urban Health
Young Adult
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
01
09
2018
accepted:
17
06
2019
entrez:
12
7
2019
pubmed:
12
7
2019
medline:
3
3
2020
Statut:
epublish
Résumé
Exposure to air pollution particulate matter (PM) and tuberculosis (TB) are two of the leading global public health challenges affecting low and middle income countries. An estimated 4.26 million premature deaths are attributable to household air pollution and an additional 4.1 million to outdoor air pollution annually. Mycobacterium tuberculosis (M.tb) infects a large proportion of the world's population with the risk for TB development increasing during immunosuppressing conditions. There is strong evidence that such immunosuppressive conditions develop during household air pollution exposure, which increases rates of TB development. Exposure to urban air pollution has been shown to alter the outcome of TB therapy. Here we examined whether in vitro exposure to urban air pollution PM alters human immune responses to M.tb. PM2.5 and PM10 (aerodynamic diameters <2.5μm, <10μm) were collected monthly from rainy, cold-dry and warm-dry seasons in Iztapalapa, a highly populated TB-endemic municipality of Mexico City with elevated outdoor air pollution levels. We evaluated the effects of seasonality and size of PM on cytotoxicity and antimycobacterial host immunity in human peripheral blood mononuclear cells (PBMC) from interferon gamma (IFN-γ) release assay (IGRA)+ and IGRA- healthy study subjects. PM10 from cold-dry and warm-dry seasons induced the highest cytotoxicity in PBMC. With the exception of PM2.5 from the cold-dry season, pre-exposure to all seasonal PM reduced M.tb phagocytosis by PBMC. Furthermore, M.tb-induced IFN-γ production was suppressed in PM2.5 and PM10-pre-exposed PBMC from IGRA+ subjects. This observation coincides with the reduced expression of M.tb-induced T-bet, a transcription factor regulating IFN-γ expression in T cells. Pre-exposure to PM10 compared to PM2.5 led to greater loss of M.tb growth control. Exposure to PM2.5 and PM10 collected in different seasons differentially impairs M.tb-induced human host immunity, suggesting biological mechanisms underlying altered M.tb infection and TB treatment outcomes during air pollution exposures.
Identifiants
pubmed: 31295271
doi: 10.1371/journal.pone.0219122
pii: PONE-D-18-25717
pmc: PMC6622489
doi:
Substances chimiques
Air Pollutants
0
Interleukin-1beta
0
Particulate Matter
0
T-Box Domain Proteins
0
T-box transcription factor TBX21
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0219122Subventions
Organisme : NIEHS NIH HHS
ID : R01 ES020382
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM055145
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES005022
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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