Structure and dynamics of human and bacterial acyl carrier proteins and their interactions with fatty acid synthesis proteins.

Acyl Carrier Protein / chemistry Amino Acid Sequence Animals Bacterial Proteins / chemistry Escherichia coli / chemistry Escherichia coli Proteins / chemistry Fatty Acid Synthase, Type II / chemistry Fatty Acids / metabolism Humans Molecular Docking Simulation Protein Conformation Protein Interaction Maps Sequence Alignment Streptomyces coelicolor / chemistry
Acyl carrier protein Acyl carrier protein synthase Dynamics Type I FAS Type II FAS

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
03 09 2019
Historique:
received: 29 06 2019
accepted: 05 07 2019
pubmed: 13 7 2019
medline: 2 6 2020
entrez: 13 7 2019
Statut: ppublish

Résumé

Acyl carrier protein (ACP) is highly conserved across taxa and plays key roles in the fatty acid synthesis system by mediating acyl group delivery and shuttling. Here, we compared the structural and dynamic features of human type Ι ACP (hACP) and Escherichia coli type II ACP (EcACP). Analysis of chemical shift perturbations upon octanoyl group attachment showed perturbations in hACP only near acyl-group attachment sites, whereas EcACP showed the perturbation at residues in the hydrophobic cavity. This difference confirmed that hACP does not sequester the acyl chain in the hydrophobic cavity, which is blocked by hydrophobic triad residues (L34, L39, and V64). Moreover, hACP showed more flexible backbone dynamics than EcACP, especially in the front of α

Identifiants

DOI: 10.1016/j.bbrc.2019.07.018 PMID: 31296387
pubmed: 31296387
pii: S0006-291X(19)31354-3
doi: 10.1016/j.bbrc.2019.07.018
pii:
doi:

Substances chimiques

Acyl Carrier Protein 0
Bacterial Proteins 0
Escherichia coli Proteins 0
Fatty Acids 0
acpP protein, E coli 0
Fatty Acid Synthase, Type II EC 6.-

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1183-1189

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Jungwoo Park (J)

Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.

Yeongjoon Lee (Y)

Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.

Dasom Cheon (D)

Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea.

Yangmee Kim (Y)

Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, South Korea. Electronic address: ymkim@konkuk.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéine ACP › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Eucaryotes › Animaux › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Acyl-carrier protein S-malonyltransferase › Fatty acid synthases › Fatty acid synthase type II › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Lipides › Acides gras › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Cartes d'interactions protéiques › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Structure and dynamics of human and bacterial...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Streptomyces coelicolor › Structure and dynamics of human and bacterial...