Nanoscale Structural Organization of Insulin Fibril Polymorphs Revealed by Atomic Force Microscopy-Infrared Spectroscopy (AFM-IR).
AFM-IR
amyloid fibrils
nanoscale imaging
oligomers
polymorphs
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
17 02 2020
17 02 2020
Historique:
received:
16
06
2019
pubmed:
13
7
2019
medline:
5
2
2021
entrez:
13
7
2019
Statut:
ppublish
Résumé
Spontaneous aggregation of misfolded proteins typically results in the formation of morphologically and structurally different amyloid fibrils, protein aggregates that are strongly associated with various neurodegenerative disorders. Elucidation of the structural organization of amyloid aggregates is crucial to understanding their role in the onset and progression of these diseases. Using atomic force microscopy-infrared spectroscopy (AFM-IR), we investigated the structural organization of insulin fibrils. We found that insulin aggregation results in the formation of two structurally different fibril polymorphs. One polymorph has a β-sheet core surrounded by primarily unordered protein secondary structure. This polymorph has β-sheet-rich surface, whereas the surface of the other fibril polymorph is primarily composed of unordered protein. Using AFM-IR, we also revealed the structural organization of the insulin oligomers. Finally, we discovered a new pathway for amyloid fibril formation that is based on a fusion of several oligomers into a single fibril structure.
Identifiants
pubmed: 31299124
doi: 10.1002/cbic.201900394
doi:
Substances chimiques
Amyloid
0
Insulin
0
Protein Aggregates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
481-485Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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