Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage.
Amino Acid Sequence
Animals
Antibodies, Viral
/ immunology
Bacteriophage M13
/ genetics
Capsid Proteins
/ genetics
Cell Surface Display Techniques
Chickens
/ immunology
Epitopes
/ genetics
Immunogenicity, Vaccine
Influenza A virus
Influenza Vaccines
/ immunology
Orthomyxoviridae Infections
/ prevention & control
Recombinant Fusion Proteins
/ immunology
Viral Matrix Proteins
/ genetics
Immunization
Influenza A virus
M13 phage
M2e (2-9)
Journal
Comparative immunology, microbiology and infectious diseases
ISSN: 1878-1667
Titre abrégé: Comp Immunol Microbiol Infect Dis
Pays: England
ID NLM: 7808924
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
21
01
2019
revised:
23
04
2019
accepted:
06
05
2019
entrez:
14
7
2019
pubmed:
14
7
2019
medline:
30
11
2019
Statut:
ppublish
Résumé
Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens.
Identifiants
pubmed: 31300131
pii: S0147-9571(19)30083-9
doi: 10.1016/j.cimid.2019.05.004
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Capsid Proteins
0
Epitopes
0
Influenza Vaccines
0
M2 protein, Influenza A virus
0
Recombinant Fusion Proteins
0
Viral Matrix Proteins
0
coat protein, Bacteriophage M13
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
76-80Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.