Frequency and expression of genes involved in adhesion and biofilm formation in Staphylococcus aureus strains isolated from periodontal lesions.
Adhesins, Bacterial
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Antigens, Bacterial
/ genetics
Biofilms
/ drug effects
Cell Line
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple, Bacterial
/ genetics
Electrophoresis, Gel, Pulsed-Field
Epithelial Cells
Female
Gene Expression Regulation, Bacterial
Genotype
Humans
Male
Mexico
Microbial Sensitivity Tests
Microbiota
Mouth
/ microbiology
Phenotype
Polymerase Chain Reaction
Staphylococcal Infections
/ microbiology
Staphylococcus aureus
/ drug effects
Virulence
/ genetics
Antibiotic-resistance
Human
In vitro infection model
Oral microbiota
Journal
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
ISSN: 1995-9133
Titre abrégé: J Microbiol Immunol Infect
Pays: England
ID NLM: 100956211
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
06
02
2019
revised:
09
04
2019
accepted:
29
05
2019
pubmed:
14
7
2019
medline:
8
10
2021
entrez:
14
7
2019
Statut:
ppublish
Résumé
The aim of this study was to characterize the Staphylococcus aureus strains isolated from periodontal lesions of patients, to determine the expression of genes involved in cell adhesion upon their infection of human epithelial cells using an in vitro model, its biofilm formation, and its resistance to antibiotics. S. aureus was analysed by PCR, Kirby-Bauer, and pulsed-field gel electrophoresis (PFGE), measuring gene expression by real-time PCR after infection of human cells in vitro. S. aureus was identified in 18.6% (50/268) of the samples. All strains (n = 50) possessed the virulence genes spa (Staphylococcal protein A), coa (coagulase), and icaAB (intercellular adhesin); 96% (n = 48) possessed clfB (clumping factor B), and 88% (n = 44) possessed ebps (elastin-binding protein) and sdrD (serine aspartate repeat protein D). All strains were resistant to methicillin, ampicillin, dicloxacillin, cefotaxime, and penicillin, and were multidrug resistant to 6-12 antibiotics. PFGE analysis showed 37 different pulsed-field types and most strains (60.4%) had a unique pulsed-field type. Twenty-four distinct combinations of virulence genes and antibiotic-resistant phenotypes were identified. Although S. aureus has been considered a transient member of the oral microbiota, our results indicate a high-level expression of virulence genes and multidrug resistance in the strains isolated from periodontal lesions. These strains might complicate the successful treatment of the disease.
Sections du résumé
BACKGROUND/PURPOSE
OBJECTIVE
The aim of this study was to characterize the Staphylococcus aureus strains isolated from periodontal lesions of patients, to determine the expression of genes involved in cell adhesion upon their infection of human epithelial cells using an in vitro model, its biofilm formation, and its resistance to antibiotics.
METHODS
METHODS
S. aureus was analysed by PCR, Kirby-Bauer, and pulsed-field gel electrophoresis (PFGE), measuring gene expression by real-time PCR after infection of human cells in vitro.
RESULTS
RESULTS
S. aureus was identified in 18.6% (50/268) of the samples. All strains (n = 50) possessed the virulence genes spa (Staphylococcal protein A), coa (coagulase), and icaAB (intercellular adhesin); 96% (n = 48) possessed clfB (clumping factor B), and 88% (n = 44) possessed ebps (elastin-binding protein) and sdrD (serine aspartate repeat protein D). All strains were resistant to methicillin, ampicillin, dicloxacillin, cefotaxime, and penicillin, and were multidrug resistant to 6-12 antibiotics. PFGE analysis showed 37 different pulsed-field types and most strains (60.4%) had a unique pulsed-field type. Twenty-four distinct combinations of virulence genes and antibiotic-resistant phenotypes were identified.
CONCLUSION
CONCLUSIONS
Although S. aureus has been considered a transient member of the oral microbiota, our results indicate a high-level expression of virulence genes and multidrug resistance in the strains isolated from periodontal lesions. These strains might complicate the successful treatment of the disease.
Identifiants
pubmed: 31300301
pii: S1684-1182(19)30076-3
doi: 10.1016/j.jmii.2019.05.010
pii:
doi:
Substances chimiques
Adhesins, Bacterial
0
Anti-Bacterial Agents
0
Antigens, Bacterial
0
streptococcal protective antigen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
267-275Informations de copyright
Copyright © 2019. Published by Elsevier B.V.