A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation.
ADCD
Antibody-dependent effector function
Complement
Fc receptor
High-throughput
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
29
12
2018
revised:
21
05
2019
accepted:
08
07
2019
pubmed:
14
7
2019
medline:
7
5
2020
entrez:
14
7
2019
Statut:
ppublish
Résumé
The complement system plays a critical role in innate immune defense against pathogens, both via non-specific direct pathogen recognition and killing or via antigen-specific indirect recruitment by complement fixing antibodies. While various assays for measuring complement activation have been developed, few provide a high-throughput, sample-sparing approach to interrogate the qualitative differences in the ability of antibodies to drive complement activation. Here we present a high-throughput, sample-sparing, bead-based assay to evaluate antigen-specific antibody-dependent complement activation against nearly any antigen. Optimization of buffer composition, kinetics of immune complex formation, as well as complement source all contribute critically to the development of a robust, highly flexible and high-throughput approach to analyze antibody-dependent complement deposition (ADCD). Thus, the optimized bead-based, antigen-specific assay represents a simple, highly adaptable platform to profile antibody-dependent complement activation across pathogens and diseases.
Identifiants
pubmed: 31301278
pii: S0022-1759(18)30463-0
doi: 10.1016/j.jim.2019.07.002
pmc: PMC6722412
pii:
doi:
Substances chimiques
Antibodies
0
Complement System Proteins
9007-36-7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112630Subventions
Organisme : NIAID NIH HHS
ID : U19 AI135995
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI060354
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
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