CLARITY analysis of the Cl/pH sensor expression in the brain of transgenic mice.

Animals Axons Brain Green Fluorescent Proteins / genetics Hydrogen-Ion Concentration Mice Mice, Transgenic Microscopy, Fluorescence
fluorescence imaging, transparent brain genetically encoded biosensors intracellular ions monitoring

Journal

Neuroscience
ISSN: 1873-7544
Titre abrégé: Neuroscience
Pays: United States
ID NLM: 7605074

Informations de publication

Date de publication:
15 07 2020
Historique:
received: 26 03 2019
revised: 01 07 2019
accepted: 02 07 2019
pubmed: 16 7 2019
medline: 15 5 2021
entrez: 15 7 2019
Statut: ppublish

Résumé

Genetically encoded biosensors are widely used in cell biology for the non-invasive imaging of concentrations of ions or the activity of enzymes, to evaluate the distribution of small molecules, proteins and organelles, and to image protein interactions in living cells. These fluorescent molecules can be used either by transient expression in cultured cells or in entire organisms or through stable expression by producing transgenic animals characterized by genetically encoded and heritable biosensors. Using the mouse Thy1 mini-promoter, we generated a line of transgenic mice expressing a genetically encoded sensor for the simultaneous measurements of intracellular Cl

Identifiants

DOI: 10.1016/j.neuroscience.2019.07.010 PMID: 31302264
pubmed: 31302264
pii: S0306-4522(19)30481-6
doi: 10.1016/j.neuroscience.2019.07.010
pii:
doi:

Substances chimiques

Green Fluorescent Proteins 147336-22-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-194

Informations de copyright

Copyright © 2019 IBRO. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that there are no conflicts of interest.

Auteurs

Artem V Diuba (AV)

Aix-Marseille University, INSERM, INS, Institut of System Neurosciences, 13005 Marseille, France; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992, Moscow, Russia.

Dmitry V Samigullin (DV)

Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 420111, Kazan, Russia; Department of Radiophotonics and microwave technologies, Kazan National Research Technical University named after A.N.Tupolev, 420111, Kazan, Russia; Open Laboratory of Neuropharmacology, Kazan Federal University,420111, Kazan, Russia.

Attila Kaszas (A)

Aix-Marseille University, INSERM, INS, Institut of System Neurosciences, 13005 Marseille, France; Institut de Neurosciences de la Timone, CNRS UMR 7289 & Aix- Marseille Université, 13005 Marseille, France.

Francesca Zonfrillo (F)

Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory, EMBL-Rome, Via Ramarini 32, 00015 Monterotondo, ITALY.

Anton Malkov (A)

Aix-Marseille University, INSERM, INS, Institut of System Neurosciences, 13005 Marseille, France; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Russia.

Elena Petukhova (E)

Institute of Neurosciences, Kazan Medical State University, Kazan, Russia.

Antonio Casini (A)

CIBIO University of Trento, Italy.

Daniele Arosio (D)

Institute of Biophysics, National Research Council of Italy, 38123 Trento, Italy.

Monique Esclapez (M)

Aix-Marseille University, INSERM, INS, Institut of System Neurosciences, 13005 Marseille, France.

Cornelius T Gross (CT)

Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory, EMBL-Rome, Via Ramarini 32, 00015 Monterotondo, ITALY.

Piotr Bregestovski (P)

Aix-Marseille University, INSERM, INS, Institut of System Neurosciences, 13005 Marseille, France; Institute of Neurosciences, Kazan Medical State University, Kazan, Russia. Electronic address: piotr.bregestovski@univ-amu.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › CLARITY analysis of the Cl/pH sensor...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › CLARITY analysis of the Cl/pH sensor...