The Molecular Architecture of Native BBSome Obtained by an Integrated Structural Approach.
BBSome
cilia
trafficking
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
03 09 2019
03 09 2019
Historique:
received:
10
04
2019
revised:
21
05
2019
accepted:
17
06
2019
pubmed:
16
7
2019
medline:
13
5
2020
entrez:
16
7
2019
Statut:
ppublish
Résumé
The unique membrane composition of cilia is maintained by a diffusion barrier at the transition zone that is breached when the BBSome escorts signaling receptors out of cilia. Understanding how the BBSome removes proteins from cilia has been hampered by a lack of structural information. Here, we present a nearly complete Cα model of BBSome purified from cow retina. The model is based on a single-particle cryo-electron microscopy density map at 4.9-Å resolution that was interpreted with the help of comprehensive Rosetta-based structural modeling constrained by crosslinking mass spectrometry data. We find that BBSome subunits have a very high degree of interconnectivity, explaining the obligate nature of the complex. Furthermore, like other coat adaptors, the BBSome exists in an autoinhibited state in solution and must thus undergo a conformational change upon recruitment to membranes by the small GTPase ARL6/BBS3. Our model provides the first detailed view of the machinery enabling ciliary exit.
Identifiants
pubmed: 31303482
pii: S0969-2126(19)30205-9
doi: 10.1016/j.str.2019.06.006
pmc: PMC6726506
mid: NIHMS1532937
pii:
doi:
Substances chimiques
Microtubule-Associated Proteins
0
ADP-Ribosylation Factors
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1384-1394.e4Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM089218
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123089
Pays : United States
Organisme : Austrian Science Fund FWF
ID : P 30162
Pays : Austria
Organisme : NIGMS NIH HHS
ID : R01 GM089933
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY002162
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
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