Mediation of antiinflammatory effects of Rg3-enriched red ginseng extract from Korean Red Ginseng via retinoid X receptor α-peroxisome-proliferating receptor γ nuclear receptors.
Antiinflammation
Ginseng
Peroxisome-proliferating receptor γ
Retinoid X receptor α
Rg3-enriched red ginseng extract
Journal
Journal of ginseng research
ISSN: 1226-8453
Titre abrégé: J Ginseng Res
Pays: Korea (South)
ID NLM: 100890690
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
12
04
2018
revised:
31
05
2018
accepted:
26
06
2018
entrez:
17
7
2019
pubmed:
17
7
2019
medline:
17
7
2019
Statut:
ppublish
Résumé
Ginseng has a wide range of beneficial effects on health, such as the mitigation of minor and major inflammatory diseases, cancer, and cardiovascular diseases. There are abundant data regarding the health-enhancing properties of whole ginseng extracts and single ginsenosides; however, no study to date has determined the receptors that mediate the effects of ginseng extracts. In this study, for the first time, we explored whether the antiinflammatory effects of Rg3-enriched red ginseng extract (Rg3-RGE) are mediated by retinoid X receptor α-peroxisome-proliferating receptor γ (RXRα-PPARγ) heterodimer nuclear receptors. Nitric oxide assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay, quantitative reverse transcription polymerase chain reaction, nuclear hormone receptor-binding assay, and molecular docking analyses were used for this study. Rg3-RGE exerted antiinflammatory effects via nuclear receptor heterodimers between RXRα and PPARγ agonists and antagonists. These findings indicate that Rg3-RGE can be considered a potent antiinflammatory agent, and these effects are likely mediated by the nuclear receptor RXRα-PPARγ heterodimer.
Sections du résumé
BACKGROUND
BACKGROUND
Ginseng has a wide range of beneficial effects on health, such as the mitigation of minor and major inflammatory diseases, cancer, and cardiovascular diseases. There are abundant data regarding the health-enhancing properties of whole ginseng extracts and single ginsenosides; however, no study to date has determined the receptors that mediate the effects of ginseng extracts. In this study, for the first time, we explored whether the antiinflammatory effects of Rg3-enriched red ginseng extract (Rg3-RGE) are mediated by retinoid X receptor α-peroxisome-proliferating receptor γ (RXRα-PPARγ) heterodimer nuclear receptors.
METHODS
METHODS
Nitric oxide assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay, quantitative reverse transcription polymerase chain reaction, nuclear hormone receptor-binding assay, and molecular docking analyses were used for this study.
RESULTS
RESULTS
Rg3-RGE exerted antiinflammatory effects via nuclear receptor heterodimers between RXRα and PPARγ agonists and antagonists.
CONCLUSION
CONCLUSIONS
These findings indicate that Rg3-RGE can be considered a potent antiinflammatory agent, and these effects are likely mediated by the nuclear receptor RXRα-PPARγ heterodimer.
Identifiants
pubmed: 31308816
doi: 10.1016/j.jgr.2018.06.005
pii: S1226-8453(18)30111-8
pmc: PMC6606843
doi:
Types de publication
Journal Article
Langues
eng
Pagination
442-451Références
Hepatology. 2001 Jan;33(1):91-9
pubmed: 11124825
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2610-5
pubmed: 11226287
J Biol Chem. 2001 Mar 23;276(12):9452-9
pubmed: 11254657
Mol Endocrinol. 2001 Aug;15(8):1360-9
pubmed: 11463859
Mol Cell Biol. 2002 Apr;22(8):2632-41
pubmed: 11909957
Nat Med. 2003 Feb;9(2):213-9
pubmed: 12524534
J Biol Chem. 2003 Mar 21;278(12):10443-9
pubmed: 12531895
Genes Dev. 1992 Mar;6(3):329-44
pubmed: 1312497
FEBS Lett. 2004 Aug 13;572(1-3):266-70
pubmed: 15304360
Cell. 2004 Oct 15;119(2):299-309
pubmed: 15479645
FEBS J. 2005 Mar;272(6):1546-56
pubmed: 15752369
J Neurosci Res. 2005 Aug 1;81(3):403-11
pubmed: 15968640
Biochem Biophys Res Commun. 2005 Oct 14;336(1):215-22
pubmed: 16125673
Annu Rev Immunol. 2007;25:101-37
pubmed: 17090225
J Neuroimmunol. 2007 Feb;183(1-2):50-9
pubmed: 17175031
Biochem J. 2008 Mar 1;410(2):319-30
pubmed: 18031289
Methods Mol Biol. 2008;443:365-82
pubmed: 18446297
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10626-31
pubmed: 20498053
Trends Endocrinol Metab. 2010 Nov;21(11):676-83
pubmed: 20674387
PLoS One. 2010 Nov 30;5(11):e15119
pubmed: 21152046
Biochim Biophys Acta. 2012 Jan;1821(1):57-69
pubmed: 21515403
Curr Comput Aided Drug Des. 2011 Jun;7(2):146-57
pubmed: 21534921
Nature. 1990 May 17;345(6272):224-9
pubmed: 2159111
Trends Endocrinol Metab. 2011 Aug;22(8):333-43
pubmed: 21646028
J Neurosci. 2011 Jun 29;31(26):9620-9
pubmed: 21715627
Biol Pharm Bull. 2012;35(1):1-9
pubmed: 22223330
Front Pharmacol. 2012 Feb 28;3:25
pubmed: 22403544
J Chem Inf Model. 2012 Jul 23;52(7):1757-68
pubmed: 22587354
Phytother Res. 2013 Jul;27(7):949-65
pubmed: 22969004
Semin Immunopathol. 2013 Mar;35(2):151-61
pubmed: 23370700
Trends Endocrinol Metab. 2013 Sep;24(9):460-8
pubmed: 23701753
Mediators Inflamm. 2013;2013:549627
pubmed: 23781121
Mol Med Rep. 2013 Dec;8(6):1611-6
pubmed: 24100795
Acta Pharmacol Sin. 2013 Nov;34(11):1367-73
pubmed: 24122014
Oncol Rep. 2014 Feb;31(2):919-25
pubmed: 24337872
Cell. 2014 Mar 27;157(1):255-66
pubmed: 24679540
Tumour Biol. 2014 Dec;35(12):11985-94
pubmed: 25168366
Oncol Rep. 2014 Nov;32(5):1803-8
pubmed: 25175462
Nat Commun. 2014 Nov 24;5:5494
pubmed: 25417649
Mol Med Rep. 2015 Sep;12(3):3557-3562
pubmed: 25998024
Biochem Biophys Res Commun. 2015 Aug 7;463(4):1184-9
pubmed: 26086107
Adv Clin Chem. 2015;71:171-203
pubmed: 26411415
Chin J Nat Med. 2016 Jan;14(1):7-16
pubmed: 26850342
Anticancer Res. 2016 Dec;36(12):6297-6301
pubmed: 27919949
J Ginseng Res. 2017 Oct;41(4):548-555
pubmed: 29021703
Genes Dev. 1995 Apr 1;9(7):769-82
pubmed: 7705655
J Biol Chem. 1998 Feb 6;273(6):3212-5
pubmed: 9452433