Label-Free Time-of-Flight Secondary Ion Mass Spectrometry Imaging of Sulfur-Producing Enzymes inside Microglia Cells following Exposure to Silver Nanowires.

Animals Biological Transport Cell Line, Transformed Cystathionine beta-Synthase / metabolism Cystathionine gamma-Lyase / metabolism Mice Microglia / drug effects Microscopy, Electron, Scanning Molecular Imaging / instrumentation Nanowires / chemistry Silver / chemistry Spectrometry, Mass, Secondary Ion Sulfur / chemistry Sulfurtransferases / metabolism

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
03 09 2019
Historique:
pubmed: 17 7 2019
medline: 29 9 2020
entrez: 17 7 2019
Statut: ppublish

Résumé

There are no methods sensitive enough to detect enzymes within cells, without the use of analyte labeling. Here we show that it is possible to detect protein ion signals of three different H

Identifiants

DOI: 10.1021/acs.analchem.9b01704 PMID: 31310103
pubmed: 31310103
doi: 10.1021/acs.analchem.9b01704
doi:

Substances chimiques

Silver 3M4G523W1G
Sulfur 70FD1KFU70
Sulfurtransferases EC 2.8.1.-
Cystathionine beta-Synthase EC 4.2.1.22
Cystathionine gamma-Lyase EC 4.4.1.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11098-11107

Auteurs

Bey Fen Leo (BF)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
Central Unit for Advanced Research Imaging (CENTUARI), Faculty of Medicine , University of Malaya , Kuala Lumpur 50603 , Malaysia.

Sarah Fearn (S)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.

Daniel Gonzalez-Cater (D)

Innovation Center of NanoMedicine , 3 Chome-25-14, Tonomachi , Kawasaki 210-0821 , Japan.
ORCID: 0000-0003-4249-7062

Ioannis Theodorou (I)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.

Pakatip Ruenraroengsak (P)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.

Angela E Goode (AE)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.

David McPhail (D)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.

David T Dexter (DT)

Innovation Center of NanoMedicine , 3 Chome-25-14, Tonomachi , Kawasaki 210-0821 , Japan.

Milo Shaffer (M)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
Department of Chemistry and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
ORCID: 0000-0001-9384-9043

Kian F Chung (KF)

Experimental Studies, National Heart & Lung Institute , Imperial College London , London SW3 6LY , U.K.
ORCID: 0000-0001-7101-1426

Alexandra E Porter (AE)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
ORCID: 0000-0002-9798-8398

Mary P Ryan (MP)

Department of Materials and London Centre for Nanotechnology , Imperial College London , Exhibition Road , London SW7 2AZ , U.K.
ORCID: 0000-0001-8582-3003

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Métabolisme › Transport biologique › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Lignée de cellules transformées › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › Hydro-lyases › Cystathionine beta-synthase › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-sulfur lyases › Cystathionine gamma-lyase › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Cellules › Névroglie › Microglie › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanofils › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Argent › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse d'ions secondaires › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Chalcogènes › Soufre › Label-Free Time-of-Flight Secondary Ion Mass...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Sulfur group transferases › Sulfurtransferases › Label-Free Time-of-Flight Secondary Ion Mass...