Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
16 07 2019
16 07 2019
Historique:
received:
19
11
2018
accepted:
31
05
2019
entrez:
18
7
2019
pubmed:
18
7
2019
medline:
11
10
2019
Statut:
epublish
Résumé
The germline is the only cellular lineage capable of transferring genetic information from one generation to the next. Intergenerational transmission of epigenetic memory through the germline, in the form of DNA methylation, has been proposed; however, in mammals this is largely prevented by extensive epigenetic erasure during germline definition. Here we report that, unlike mammals, the continuously-defined 'preformed' germline of zebrafish does not undergo genome-wide erasure of DNA methylation during development. Our analysis also uncovers oocyte-specific germline amplification and demethylation of an 11.5-kb repeat region encoding 45S ribosomal RNA (fem-rDNA). The peak of fem-rDNA amplification coincides with the initial expansion of stage IB oocytes, the poly-nucleolar cell type responsible for zebrafish feminisation. Given that fem-rDNA overlaps with the only zebrafish locus identified thus far as sex-linked, we hypothesise fem-rDNA expansion could be intrinsic to sex determination in this species.
Identifiants
pubmed: 31311924
doi: 10.1038/s41467-019-10894-7
pii: 10.1038/s41467-019-10894-7
pmc: PMC6635516
doi:
Substances chimiques
DNA, Ribosomal
0
RNA, Ribosomal
0
RNA, ribosomal, 45S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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