Highly sensitive chimerism detection in blood is associated with increased risk of relapse after allogeneic hematopoietic cell transplantation in childhood leukemia.
Adolescent
Biomarkers
/ blood
Child
Child, Preschool
Female
Hematopoietic Stem Cell Transplantation
Humans
Infant
Leukemia, Myeloid, Acute
/ blood
Longitudinal Studies
Male
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/ blood
Recurrence
Retrospective Studies
Risk Assessment
Transplantation Chimera
Transplantation, Homologous
allogeneic hematopoietic cell transplantation
childhood leukemia
chimerism
relapse
Journal
Pediatric transplantation
ISSN: 1399-3046
Titre abrégé: Pediatr Transplant
Pays: Denmark
ID NLM: 9802574
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
14
11
2018
revised:
04
05
2019
accepted:
19
06
2019
pubmed:
18
7
2019
medline:
26
8
2020
entrez:
18
7
2019
Statut:
ppublish
Résumé
Analysis of chimerism in blood post-HCT using STR-PCR is routinely applied in parallel with quantification of MRD to predict relapse of leukemia. RQ-PCR chimerism is 10- to 100-fold more sensitive, but clinical studies in children are sparse. We analyzed IMC in blood samples following transplantation for acute lymphoblastic or myeloid leukemia in 56 children. IMC was defined as a minimum increase of (a) 0.1% or (b) 0.05% recipient DNA between two samples. The risk of relapse was higher in children with IMC of both 0.1% and 0.05% compared to children without IMC (HR 12.8 [95% CI: 3.9-41.4; P < .0001] and 7.6 [95% CI: 2.2-26.9; P < .01], respectively). The first IMC was detected at a median of 208 days prior to relapse. The 5-year cumulative incidence of relapse for children with a single IMC was 45.5% (CI 12.3-74.4) and 41.0% (14.2-66.6) for IMC above 0.1% and 0.05%, respectively. However, in 47 and 38 children never attaining IMC > 0.1% and >0.05%, 10 and 8 children relapsed, respectively. In a landmark analysis, no association was found between IMC prior to 90 days post-HCT and subsequent relapse by either classification of IMC and AUC for RQ-PCR chimerism was 54.2% (95 CI 27.7- 84.8). Although limited by a retrospective design, these results indicate that monitoring of RQ-PCR chimerism in peripheral blood may have a role in early detection of relapse in acute childhood leukemia.
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13549Informations de copyright
© 2019 Wiley Periodicals, Inc.
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