CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
02
04
2019
accepted:
28
05
2019
entrez:
18
7
2019
pubmed:
18
7
2019
medline:
19
2
2020
Statut:
epublish
Résumé
Embryoid bodies (EBs) are three dimensional aggregates of pluripotent stem cells primarily used to investigate morphogenesis and cell toxicity, are also attractive tools in regenerative medicine. While embryonic stem cells (ESCs) and induced pluripotent cells (IPSCs) have been shown to form EBs in mouse, primate and humans, EB formation have not been previously demonstrated in mesenchymal stem cells (MSCs). Here we show that rat MSCs form EBs; which express regulatory T cell (Treg) marker Foxp3 and CC chemokine CCL1 receptor CCR8. We show a novel method for formation of EBs from MSCs under stress and demonstrate that the induction of FoxP3+ CCR8+ EBs is dependent upon CCL1 gradients which mediate cell proliferation, migration and invasion of mTregs. The identification of EBs and novel FoxP3+ CCR8+ regulatory T cells (mTregs) for selective conversion and isolation of bone marrow derived MSCs offers novel avenues for research, diagnosis and treatment.
Identifiants
pubmed: 31314754
doi: 10.1371/journal.pone.0218944
pii: PONE-D-19-09003
pmc: PMC6636727
doi:
Substances chimiques
Chemokine CCL1
0
Forkhead Transcription Factors
0
Foxp3 protein, rat
0
Receptors, CCR8
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0218944Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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