Cortical layer-specific critical dynamics triggering perception.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
09 08 2019
09 08 2019
Historique:
received:
01
01
2019
accepted:
02
07
2019
pubmed:
20
7
2019
medline:
21
3
2020
entrez:
20
7
2019
Statut:
ppublish
Résumé
Perceptual experiences may arise from neuronal activity patterns in mammalian neocortex. We probed mouse neocortex during visual discrimination using a red-shifted channelrhodopsin (ChRmine, discovered through structure-guided genome mining) alongside multiplexed multiphoton-holography (MultiSLM), achieving control of individually specified neurons spanning large cortical volumes with millisecond precision. Stimulating a critical number of stimulus-orientation-selective neurons drove widespread recruitment of functionally related neurons, a process enhanced by (but not requiring) orientation-discrimination task learning. Optogenetic targeting of orientation-selective ensembles elicited correct behavioral discrimination. Cortical layer-specific dynamics were apparent, as emergent neuronal activity asymmetrically propagated from layer 2/3 to layer 5, and smaller layer 5 ensembles were as effective as larger layer 2/3 ensembles in eliciting orientation discrimination behavior. Population dynamics emerging after optogenetic stimulation both correctly predicted behavior and resembled natural internal representations of visual stimuli at cellular resolution over volumes of cortex.
Identifiants
pubmed: 31320556
pii: science.aaw5202
doi: 10.1126/science.aaw5202
pmc: PMC6711485
mid: NIHMS1046972
pii:
doi:
Substances chimiques
Channelrhodopsins
0
Opsins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIMH NIH HHS
ID : R37 MH075957
Pays : United States
Organisme : NIDA NIH HHS
ID : P50 DA042012
Pays : United States
Organisme : NIDA NIH HHS
ID : R13 DA041796
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIDA NIH HHS
ID : R37 DA035377
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Références
Nature. 2001 Aug 2;412(6846):549-53
pubmed: 11484056
Nature. 2005 Feb 24;433(7028):868-73
pubmed: 15729343
Nature. 2009 Feb 26;457(7233):1133-6
pubmed: 19151698
Neuron. 2009 Feb 26;61(4):635-48
pubmed: 19249282
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5389-93
pubmed: 19276119
Neuron. 2009 Mar 12;61(5):700-7
pubmed: 19285467
Nat Neurosci. 2009 May;12(5):655-63
pubmed: 19377473
Nat Neurosci. 2010 Mar;13(3):344-52
pubmed: 20098420
Neuron. 2010 Aug 26;67(4):562-74
pubmed: 20797534
Nature. 2011 May 5;473(7345):87-91
pubmed: 21478872
Neuron. 2011 Apr 28;70(2):200-27
pubmed: 21521609
Front Synaptic Neurosci. 2011 Aug 29;3:4
pubmed: 22007168
Neuron. 2011 Dec 22;72(6):1025-39
pubmed: 22196337
Neuron. 2011 Dec 22;72(6):1040-54
pubmed: 22196338
Prog Neurobiol. 2013 Apr;103:41-75
pubmed: 22472964
J Neurosci. 2012 Jul 11;32(28):9716-26
pubmed: 22787057
Nat Methods. 2012 Dec;9(12):1171-9
pubmed: 23169303
Nat Neurosci. 2013 Feb;16(2):219-26
pubmed: 23292681
Nature. 2013 Apr 4;496(7443):96-100
pubmed: 23552948
Nature. 1990 Jul 12;346(6280):174-7
pubmed: 2366872
Nat Neurosci. 2013 Jul;16(7):824-31
pubmed: 23799475
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Nat Neurosci. 2013 Sep;16(9):1315-23
pubmed: 23933748
Nature. 2013 Nov 7;503(7474):51-8
pubmed: 24201278
Cell. 2014 Mar 13;156(6):1139-1152
pubmed: 24630718
Nat Rev Neurosci. 2014 May;15(5):313-25
pubmed: 24739786
PLoS Biol. 2014 Jun 24;12(6):e1001889
pubmed: 24959919
Nat Neurosci. 2014 Dec;17(12):1816-24
pubmed: 25402854
Nat Methods. 2015 Feb;12(2):140-6
pubmed: 25532138
Nat Neurosci. 2015 Feb;18(2):170-81
pubmed: 25622573
Nature. 2015 Feb 19;518(7539):399-403
pubmed: 25652823
Neuron. 2015 Jun 17;86(6):1478-90
pubmed: 26051421
Science. 2015 Jun 19;348(6241):1352-5
pubmed: 26089513
Science. 2015 Jul 3;349(6243):70-4
pubmed: 26138975
Neuron. 2015 Dec 16;88(6):1253-1267
pubmed: 26671462
Opt Express. 2015 Dec 14;23(25):32573-81
pubmed: 26699047
Nature. 2016 Apr 21;532(7599):370-4
pubmed: 27018655
Exp Neurol. 2017 Jan;287(Pt 4):437-451
pubmed: 27511294
Science. 2016 Aug 12;353(6300):691-4
pubmed: 27516599
Elife. 2016 Aug 15;5:
pubmed: 27525487
Photochem Photobiol. 2017 May;93(3):782-795
pubmed: 28500713
Neuron. 2017 May 17;94(4):774-789.e5
pubmed: 28521132
J Biol Chem. 2017 Aug 25;292(34):14205-14216
pubmed: 28659342
Curr Biol. 2018 Jan 8;28(1):114-120.e5
pubmed: 29276127
Elife. 2018 Feb 07;7:
pubmed: 29412138
Science. 2018 May 4;360(6388):537-542
pubmed: 29567809
Neuron. 2018 Apr 4;98(1):222-234.e8
pubmed: 29576389
Nat Neurosci. 2018 Jun;21(6):881-893
pubmed: 29713079
Science. 2018 May 4;360(6388):493-494
pubmed: 29724943
Nat Commun. 2018 Jul 3;9(1):2596
pubmed: 29968709
Elife. 2018 Sep 17;7:
pubmed: 30222107
Nature. 2019 Jan;565(7741):645-649
pubmed: 30651638
Nature. 2019 Mar;567(7748):334-340
pubmed: 30842660
Science. 2019 Apr 19;364(6437):253
pubmed: 30948440
J Neurosci. 1982 Jan;2(1):32-48
pubmed: 7054394
Science. 1995 Aug 18;269(5226):981-5
pubmed: 7638624