Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 07 2019
18 07 2019
Historique:
received:
18
04
2018
accepted:
28
05
2019
entrez:
20
7
2019
pubmed:
20
7
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking the retinal chromophore, as a first manifestation of multiphoton effects. We resolve distinct stages in the structural dynamics of the all-trans retinal in photoexcited bR to a highly twisted 13-cis conformation. Other active site sub-picosecond rearrangements include correlated vibrational motions of the electronically excited retinal chromophore, the surrounding amino acids and water molecules as well as their hydrogen bonding network. These results show that this extended photo-active network forms an electronically and vibrationally coupled system in bR, and most likely in all retinal proteins.
Identifiants
pubmed: 31320619
doi: 10.1038/s41467-019-10758-0
pii: 10.1038/s41467-019-10758-0
pmc: PMC6639342
doi:
Substances chimiques
Water
059QF0KO0R
Bacteriorhodopsins
53026-44-1
Retinaldehyde
RR725D715M
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3177Subventions
Organisme : NCRR NIH HHS
ID : P41 RR001209
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103393
Pays : United States
Commentaires et corrections
Type : CommentIn
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